Possible Involvement of a Single Histidine Residue in the P-Type Na+-ATPase of a Facultatively Anaerobic Alkaliphile, Exiguobacterium aurantiacum

Autor: Atsuko Hirano, Noriyuki Koyama
Rok vydání: 2001
Předmět:
Zdroj: Current Microbiology. 43:400-402
ISSN: 1432-0991
0343-8651
Popis: Effect of various inhibitors on the P-type Na+-ATPase of a facultatively anaerobic alkaliphile, Exiguobacterium aurantiacum, was examined. The ATPase was extremely sensitive to p-chloromercuriphenylsulfonic acid, a modifier of SH-group. The enzyme was also sensitive to diethylpyrocarbonate, and analysis of the inhibition kinetics by the drug indicated that modification of a single histidine residue per ATPase molecule was sufficient to inactivate the enzyme.
Databáze: OpenAIRE