Possible Involvement of a Single Histidine Residue in the P-Type Na+-ATPase of a Facultatively Anaerobic Alkaliphile, Exiguobacterium aurantiacum
Autor: | Atsuko Hirano, Noriyuki Koyama |
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Rok vydání: | 2001 |
Předmět: |
Adenosine Triphosphatases
chemistry.chemical_classification Histidine residue biology ATPase General Medicine Hydrogen-Ion Concentration Exiguobacterium aurantiacum Applied Microbiology and Biotechnology Microbiology Drug indicated Bacteria Anaerobic Enzyme chemistry Biochemistry Diethyl Pyrocarbonate biology.protein Alkaliphile Histidine Enzyme Inhibitors 4-Chloromercuribenzenesulfonate Cation Transport Proteins Anaerobic exercise Na atpase |
Zdroj: | Current Microbiology. 43:400-402 |
ISSN: | 1432-0991 0343-8651 |
Popis: | Effect of various inhibitors on the P-type Na+-ATPase of a facultatively anaerobic alkaliphile, Exiguobacterium aurantiacum, was examined. The ATPase was extremely sensitive to p-chloromercuriphenylsulfonic acid, a modifier of SH-group. The enzyme was also sensitive to diethylpyrocarbonate, and analysis of the inhibition kinetics by the drug indicated that modification of a single histidine residue per ATPase molecule was sufficient to inactivate the enzyme. |
Databáze: | OpenAIRE |
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