Ontogeny of renin gene expression in the chicken, Gallus gallus
| Autor: | Hiroko Nishimura, Eishin Yaoita, R. Ariel Gomez, Jess Hoy, Theodore C. Mehalic, Maria Luisa S. Sequeira-Lopez, Robert L. Paxton |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
animal structures Organogenesis Ontogeny 030209 endocrinology & metabolism Chick Embryo In situ hybridization Article Renin-Angiotensin System 03 medical and health sciences 0302 clinical medicine Endocrinology biology.animal Internal medicine Renin Renin–angiotensin system medicine Animals RNA Messenger 030304 developmental biology 0303 health sciences Messenger RNA biology Vertebrate Embryo Juxtaglomerular apparatus Juxtaglomerular Apparatus Real-time polymerase chain reaction medicine.anatomical_structure Gene Expression Regulation embryonic structures Animal Science and Zoology Chickens |
| Zdroj: | Gen Comp Endocrinol |
| ISSN: | 0016-6480 |
| Popis: | Renin or a renin-like enzyme evolved in ancestral vertebrates and is conserved along the vertebrate phylogeny. The ontogenic development of renin, however, is not well understood in nonmammalian vertebrates. We aimed to determine the expression patterns and relative abundance of renin mRNA in pre- and postnatal chickens (Gallus gallus, White Leghorn breed). Embryonic day 13 (E13) embryos show renal tubules, undifferentiated mesenchymal structures, and a small number of developing glomeruli. Maturing glomeruli are seen in post-hatch day 4 (D4) and day 30 (D30) kidneys, indicating that nephrogenic activity still exists in kidneys of 4-week-old chickens. In E13 embryos, renin mRNA measured by quantitative polymerase chain reaction in the adrenal glands is equivalent to the expression in the kidneys, whereas in post-hatch D4 and D30 maturing chicks, renal renin expressions increased 2-fold and 11-fold, respectively. In contrast, relative renin expression in the adrenals became lower than in the kidneys. Furthermore, renin expression is clearly visible by in situ hybridization in the juxtaglomerular (JG) area in D4 and D30 chicks, but not in E13 embryos. The results suggest that in chickens, renin evolved in both renal and extrarenal organs at an early stage of ontogeny and, with maturation, became localized to the JG area. Clear JG structures are not morphologically detectable in E13 embryos, but are visible in 30-day-old chicks, supporting this concept. |
| Databáze: | OpenAIRE |
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