Interleukin-10 Does Not Augment Osseous Regeneration in the Scarred Calvarial Defect Achieved with Low-Dose Biopatterned BMP2
| Autor: | Joseph E. Losee, Phil G. Campbell, Lee E. Weiss, Saigopalakrishna S. Yerneni, James J. Cray, Michael R. Bykowski, Jack E. Brooker, Liliana Camison, Mark P. Mooney, Gregory M. Cooper |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Staphylococcus aureus medicine.medical_specialty Bone Regeneration Bone Morphogenetic Protein 2 Inflammation Calvaria Bone morphogenetic protein 2 Surgical Flaps Cicatrix medicine Animals business.industry Regeneration (biology) Skull Histology Staphylococcal Infections Interleukin-10 Surgery Drug Combinations Interleukin 10 medicine.anatomical_structure Rabbits medicine.symptom Augment Tomography X-Ray Computed business |
| Zdroj: | Plastic and Reconstructive Surgery. 143:1215e-1223e |
| ISSN: | 0032-1052 |
| DOI: | 10.1097/prs.0000000000005640 |
| Popis: | BACKGROUND Large calvarial defects represent a major reconstructive challenge, as they do not heal spontaneously. Infection causes inflammation and scarring, further reducing the healing capacity of the calvaria. Bone morphogenetic protein-2 (BMP2) has been shown to stimulate osteogenesis but has significant side effects in high doses. BMP2 has not been tested in combination with antiinflammatory cytokines such as interleukin-10. METHODS Sixteen New Zealand White rabbits underwent 15 × 15-mm flap calvarectomies. The flap was incubated in Staphylococcus aureus and replaced, and infection and scarring were allowed to develop. The flap was subsequently removed and the wound debrided. A 15 × 15-mm square of acellular dermal matrix biopatterned with low-dose BMP2, interleukin-10, or a combination was implanted. Computed tomographic scans were taken over 42 days. Rabbits were then killed and histology was performed. RESULTS Defects treated with BMP2 showed significantly (p < 0.05) greater osseous regeneration than untreated controls. Interleukin-10 did not significantly augment the healing achieved with BMP2, and interleukin-10 alone did not significantly increase healing compared with controls. Histology showed evidence of bone formation in defects treated with BMP2. Untreated controls and defects treated with interleukin-10 alone showed only fibrous tissue in the defect site. CONCLUSIONS Low-dose BMP2 delivered directly to the scarred calvarial defect augments bony healing. Interleukin-10 at the dose applied did not significantly augment healing alone or in combination with BMP2. Healing had not finished at 42 days and analysis at later time points or the use of higher doses of BMP2 may yield greater healing. |
| Databáze: | OpenAIRE |
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