Antibacterial activity of new peptide from bovine casein hydrolyzed by a serine metalloprotease of Lactococcus lactis subsp lactis BR16
| Autor: | Mickaël Chevalier, Rémi Przybylski, Nedjar Naima, Abdelkader Dilmi-Bouras, Rafik Balti, Faiza Adoui, Pascal Dhulster, Hacene Elhameur, Christophe Flahaut, Fateh Bougherra |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Antibacterial peptide 030106 microbiology Antimicrobial peptides Medicine (miscellaneous) Peptide medicine.disease_cause Bovine casein Microbiology 03 medical and health sciences Casein medicine Serine metalloprotease TX341-641 Escherichia coli chemistry.chemical_classification Nutrition and Dietetics biology Nutrition. Foods and food supply Lactococcus lactis Adsorption/desorption biology.organism_classification Lactococcus lactis subsp lactis BR16 030104 developmental biology Biochemistry chemistry Micrococcus luteus Antibacterial activity Bacteria Food Science |
| Zdroj: | Journal of Functional Foods, Vol 32, Iss, Pp 112-122 (2017) |
| ISSN: | 1756-4646 |
| Popis: | An extracellular serine metalloprotease produced by Lactococcus lactis subsp lactis BR16 isolated from Algerian traditional fermented food was used for hydrolyzing bovine casein in order to identify antibacterial peptide. Antibacterial peptide was separated from peptic hydrolysates by adsorption/desorption then analyzed using reverse phase high performance liquid chromatography (RP-HPLC) and active fraction was analyzed by MALDI-TOF mass spectrometry. A new antibacterial peptide was identified as SSSEESII from the subunit αs2 of casein corresponding of the fragment (24–31). This peptide demonstrated antibacterial activity against two Gram-positive bacteria: Listeria innocua ATCC 33090 and Micrococcus luteus ATCC 4698, and two Gram-negative bacteria: Escherichia coli ATCC 25922 and Salmonella enteritidis ATCC13076. Prediction of peptide secondary structure indicated that this peptide is anionic and must have random coil structure with a small region of hydrophobicity, which confers to the peptide unusual behavior compared to conventional antimicrobial peptides. |
| Databáze: | OpenAIRE |
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