Comprehensive Transcriptomic Analysis of Auxin Responses in Submerged Rice Coleoptile Growth

Autor: Chin-Ying Yang, Yu-Sian Wu
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
hormone
Down-Regulation
Biology
01 natural sciences
Article
Catalysis
lcsh:Chemistry
Electron Transport
Inorganic Chemistry
Transcriptome
03 medical and health sciences
Auxin
Triiodobenzoic Acids
rice coleoptile
Physical and Theoretical Chemistry
Secondary metabolism
lcsh:QH301-705.5
Molecular Biology
Gene
transcriptomic analysis
Spectroscopy
Plant Proteins
chemistry.chemical_classification
submergence
Oryza sativa
Indoleacetic Acids
Carbohydrate Metabolism Pathway
Gene Expression Profiling
Organic Chemistry
food and beverages
Oryza
General Medicine
Mitochondria
Up-Regulation
Computer Science Applications
Cell biology
030104 developmental biology
Coleoptile
lcsh:Biology (General)
lcsh:QD1-999
chemistry
Carbohydrate Metabolism
Signal transduction
Cotyledon
Signal Transduction
auxin signaling
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 21, Iss 4, p 1292 (2020)
Volume 21
Issue 4
ISSN: 1422-0067
DOI: 10.3390/ijms21041292
Popis: Cultivating rice in wet or water direct seeding systems is simple and time and labor efficient. Rice (Oryza sativa) seeds are a unique cereal that can germinate not only when submerged, but also in anoxic conditions. Many complicated hormone signals interact in submerged seed germination. Ethylene is involved in rice coleoptile elongation, but little is known regarding the role of auxin signaling under submergence. This study demonstrated that the coleoptile is shorter and curlier when submerged with 2,3,5-triiodobenzoic acid (TIBA). In transcriptomic analysis, 3448 of the 31,860 genes were upregulated, and 4360 genes were downregulated with submergence and TIBA treatment. The Gene Ontology function classification results demonstrated that upregulated differentially expressed genes (DEGs) were mainly involved in redox, stress, and signal transduction, whereas the down-regulated DEGs were mainly involved in RNA transcription, stress, and development. Furthermore, auxin signaling involved in the carbohydrate metabolism pathway was demonstrated while using transcriptomic analysis and confirmed in a quantitative real-time polymerase chain reaction. In addition, the transcript levels of development-related genes and mitochondria-electron- transport-related genes were regulated by auxin signaling under submergence. Auxin signaling was not only involved in regulating rice coleoptile elongation and development, but also regulated secondary metabolism, carbohydrate metabolism, and mitochondria electron transport under submergence. Our results presented that auxin signaling plays an important role during rice coleoptile elongation upon the submergence condition and improving the advance of research of direct rice seeding system.
Databáze: OpenAIRE
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