Catalpol inhibits the proliferation, migration and metastasis of HCC cells by regulating miR‑140‑5p expression
| Autor: | Haoxia Li, Xiaoqiang Wu, Fangping Wang, Shengyou Chen, Xiaomin Chen, Linsheng Wu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research Carcinoma Hepatocellular Epithelial-Mesenchymal Transition microRNA-140-5p Cell Survival proliferation Iridoid Glucosides Cell catalpol epithelial-mesenchymal transformation Vimentin Cell morphology Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Antigens CD Cell Movement Cell Line Tumor Genetics medicine Humans Viability assay HCC Molecular Biology Cell Proliferation Oncogene biology Chemistry Cell growth Liver Neoplasms Articles Cell cycle Cadherins Catalpol Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Cancer research biology.protein Molecular Medicine |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2020.11667 |
| Popis: | Hepatocellular carcinoma (HCC) is a frequent malignant tumor. Catalpol is a Chinese medicine extract with a number of pharmacologically active properties. The present study aimed to investigate the effects and mechanisms of catalpol in HCC. HCC cells were treated with catalpol in the presence or absence of microRNA (miR)‑140‑5p inhibitor, and assays to determine cell viability, proliferation, invasion and migration were performed. Reverse transcription‑quantitative PCR and western blotting were performed to determine the mRNA and protein expression levels of miR‑140‑5p, vimentin, N‑Cadherin and E‑Cadherin. Moreover, cells were treated with catalpol in the absence or presence of transforming growth factor (TGF)‑β1, and the cell morphology was observed under a microscope. The results demonstrated that catalpol inhibited cell proliferation, invasion and migration, and decreased the expression levels of vimentin and N‑cadherin, but increased the expression levels of E‑cadherin and miR‑140‑5p. Catalpol inhibited morphological changes in epithelial‑mesenchymal transformation (EMT) of cells induced by TGF‑β1. Following inhibition of miR‑140‑5p expression, the proliferation, invasion and migration of HCC cells were promoted, E‑cadherin expression was decreased, and the levels of vimentin and N‑cadherin were increased. The miR‑140‑5p inhibitor effectively reversed the inhibitory effect of catalpol on cell proliferation, invasion and migration. Thus, the results suggested that the antitumor potential of catalpol in HCC may be exerted by regulating the expression of miR‑140‑5p to inhibit proliferation, invasion, migration and EMT of HCC cells. |
| Databáze: | OpenAIRE |
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