Parallel up-regulation of the profilin gene family following independent domestication of diploid and allopolyploid cotton ( Gossypium )
| Autor: | Zining Wang, Armel Salmon, Guanjing Hu, Lex E. Flagel, Ying Bao, Magdalena Bezanilla, Andrew H. Paterson, Jonathan F. Wendel |
|---|---|
| Přispěvatelé: | Qufu Normal University, Iowa State University Department of Botany, Iowa State University (ISU), Duke University [Durham], Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Department of Biology, University of Massachusetts System (UMASS), Plant Genome Mapping Laboratory, University of Georgia [USA], NSF Plant Genome Program, Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
Molecular Sequence Data directional selection Real-Time Polymerase Chain Reaction Gossypium 01 natural sciences Profilins 03 medical and health sciences Species Specificity Gene Expression Regulation Plant Gene family Selection Genetic Domestication Gene polyploidy Phylogeny 030304 developmental biology 2. Zero hunger Genetics Likelihood Functions 0303 health sciences Ploidies Multidisciplinary Base Sequence Models Genetic biology Actin monomer binding Sequence Analysis DNA Biological Sciences Microarray Analysis biology.organism_classification Biological Evolution Gene expression profiling Profilin crop evolution biology.protein [SDE.BE]Environmental Sciences/Biodiversity and Ecology Ploidy fiber 010606 plant biology & botany |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2011, 108 (52), pp.21152-21157. ⟨10.1073/pnas.1115926109⟩ Proceedings of the National Academy of Sciences of the United States of America, 2011, 108 (52), pp.21152-21157. ⟨10.1073/pnas.1115926109⟩ |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1115926109 |
| Popis: | Cotton is remarkable among our major crops in that four species were independently domesticated, two allopolyploids and two diploids. In each case thousands of years of human selection transformed sparsely flowering, perennial shrubs into highly productive crops with seeds bearing the vastly elongated and abundant single-celled hairs that comprise modern cotton fiber. The genetic underpinnings of these transformations are largely unknown, but comparative gene expression profiling experiments have demonstrated up-regulation of profilin accompanying domestication in all three species for which wild forms are known. Profilins are actin monomer binding proteins that are important in cytoskeletal dynamics and in cotton fiber elongation. We show that Gossypium diploids contain six profilin genes ( GPRF 1– GPRF 6), located on four different chromosomes (eight chromosomes in the allopolyploid). All but one profilin ( GPRF 6) are expressed during cotton fiber development, and both homeologs of GPRF 1– GPRF 5 are expressed in fibers of the allopolyploids. Remarkably, quantitative RT-PCR and RNAseq data demonstrate that GPRF 1– GPRF 5 are all up-regulated, in parallel, in the three independently domesticated cottons in comparison with their wild counterparts. This result was additionally supported by iTRAQ proteomic data. In the allopolyploids, there This usage of novel should be fine, since it refers to a novel evolutionary process, not a novel discovery has been novel recruitment of the sixth profilin gene ( GPRF 6) as a result of domestication. This parallel up-regulation of an entire gene family in multiple species in response to strong directional selection is without precedent and suggests unwitting selection on one or more upstream transcription factors or other proteins that coordinately exercise control over profilin expression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|