Pro-inflammatory Cytokines Are Involved in Fluoride-Induced Cytotoxic Potential in HeLa Cells
| Autor: | Hong-wei Wang, Jing Zhao, Pan-pan Tan, Bian-hua Zhou, Wen-peng Zhao, Jian-wen Cao |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Endocrinology
Diabetes and Metabolism Clinical Biochemistry 010501 environmental sciences Biology 01 natural sciences Biochemistry Proinflammatory cytokine Inorganic Chemistry HeLa 03 medical and health sciences Fluorides 0302 clinical medicine Cytotoxic T cell Humans Cytotoxicity 0105 earth and related environmental sciences Cell Proliferation chemistry.chemical_classification Dose-Response Relationship Drug Cell growth Cytotoxins Biochemistry (medical) General Medicine biology.organism_classification Molecular biology Reverse transcriptase Enzyme chemistry Gene Expression Regulation 030220 oncology & carcinogenesis Cytokines Tumor necrosis factor alpha HeLa Cells |
| Zdroj: | Biological trace element research. 175(1) |
| ISSN: | 1559-0720 |
| Popis: | This study was designed to investigate the pro-inflammatory cytokines and their involvement in the cytotoxic potential of fluoride (F) in HeLa cells. HeLa cells were cultured with varying F concentrations (1-50 mg/L) for 48 h, and treatment effects were analyzed. The viability of HeLa cells was determined with a colorimetric method. The concentrations of IL-1β, IL-2, IL-6, and TNF-a in culture supernatant were measured through enzyme linked immunosorbent assay (ELISA). The mRNA expression levels of IL-1β, IL-2, IL-6 and TNF-a were subjected to transcript analysis and quantified through reverse transcription real-time PCR. Results showed that 10, 20 and 50 mg/L F significantly decreased the viability of HeLa cells incubated for 24 and 48 h. With their cytotoxic effect, the concentrations of IL-1β, IL-2, IL-6, and TNF-a decreased significantly in response to F, especially at 20 and 50 mg/L for 48 h. The mRNA expression levels of IL-1β, IL-2, IL-6, and TNF-a were downregulated at 50 mg/L F for 48 h. Therefore, F inhibited HeLa cell growth; as such, F could be used to alleviate the inhibition of pro-inflammatory cytokine expression. |
| Databáze: | OpenAIRE |
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