Ultrastructural comparison of dendritic spine morphology preserved with cryo and chemical fixation
| Autor: | Jerome Blanc, Natalya Korogod, Hiromi Tamada, Graham Knott, Carl C.H. Petersen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Dendritic spine geometry Tissue Fixation Mouse Brain tissue stimulation law.invention Mice 0302 clinical medicine law ampa Biology (General) Fixation (histology) 0303 health sciences dendritic spine Chemistry General Neuroscience Brain General Medicine Anatomy organization musculoskeletal system Fixation method ultrastructure cryo fixation cortex Medicine Artifacts musculoskeletal diseases extracellular-space QH301-705.5 Spine neck Science Dendritic Spines serial electron-microscopy General Biochemistry Genetics and Molecular Biology dendrite 03 medical and health sciences Fixatives Spine head Animals 030304 developmental biology Cryopreservation synaptic plasticity General Immunology and Microbiology electron microscopy stability neck Weak correlation Mice Inbred C57BL Synaptic plasticity Ultrastructure Electron microscope Research Advance 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | eLife eLife, Vol 9 (2020) |
| ISSN: | 2050-084X |
| Popis: | Previously we showed that cryo fixation of brain tissue gave a truer representation of brain ultrastructure in comparison with a standard chemical fixation method (Korogod et al 2005). Extracellular space matched physiological measurements, there were larger numbers of docked vesicles and less glial coverage of synapses and blood capillaries. Here, using the same preservation approaches we compared the morphology of dendritic spines. We show that the length of the spine and the volume of its head is unchanged, however, the spine neck width is thinner by more than 30 % after cryo fixation. In addition, the weak correlation between spine neck width and head volume seen after chemical fixation was not present in cryo-fixed spines. Our data suggest that spine neck geometry is independent of the spine head volume, with cryo fixation showing enhanced spine head compartmentalization and a higher predicted electrical resistance between spine head and parent dendrite. |
| Databáze: | OpenAIRE |
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