Hypocotyl Elongation Inhibition of Melatonin Is Involved in Repressing Brassinosteroid Biosynthesis in Arabidopsis
Autor: | Fangjie Xiong, Fengping Zhuo, Russel J. Reiter, Lingling Wang, Zhenzhen Wei, Kexuan Deng, Yun Song, Ghulam Qanmber, Li Feng, Zuoren Yang, Fuguang Li, Maozhi Ren |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Mutant Arabidopsis melatonin Plant Science lcsh:Plant culture Hypocotyl Melatonin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Brassinosteroid lcsh:SB1-1110 Original Research Brassinolide biology Chemistry fungi biology.organism_classification Cell biology 030104 developmental biology gene expression hypocotyl inhibition Plant hormone Signal transduction BR biosynthesis 030217 neurology & neurosurgery hormones hormone substitutes and hormone antagonists medicine.drug |
Zdroj: | Frontiers in Plant Science, Vol 10 (2019) Frontiers in Plant Science |
Popis: | Melatonin functions as a plant hormone/regulator in the regulation of growth and development. However, the underlying mechanisms are still unclear. In this study, we found that a high dose of melatonin inhibited hypocotyl elongation in a dose-dependent manner in Arabidopsis. An expression profile analysis showed that hypocotyl growth inhibition by melatonin was involved in reprograming the expression of cell elongation genes and brassinosteroid (BRs) biosynthetic genes. Furthermore, similar to BR biosynthetic inhibitor brassinazole (BRZ), a high concentration of melatonin upregulated BR-biosynthetic genes and downregulated BR-induced genes involved in cell elongation, while melatonin was inefficient in brassinazole-resistant mutants like the bzr1-1D and bes1-D in hypocotyl inhibition. The comparative expression profile analysis showed an opposite expression mode in the co-regulated genes between melatonin and BZR1 or melatonin and brassinolide (BL). Additionally, exogenous BL rescued the repressive phenotype of BR biosynthesis-deficient mutant like det2-1 even in the presence of high-dose melatonin, but not BR receptor mutant bri1-5 or signal transduction mutant bin2-1. A biochemical analysis further confirmed that melatonin reduced endogenous BR levels in a dose-dependent manner in Arabidopsis. Taken together, these results indicate that melatonin inhibits BR biosynthesis but does not block BR signaling in the inhibition of hypocotyl elongation and extends insights on the role of melatonin in cross-talking with plant hormone signaling. |
Databáze: | OpenAIRE |
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