Genotoxicity and repeated-dose toxicity evaluation of dried Wolffia globosa Mankai
| Autor: | Tomoko Shimada, Yasuko Kawamata, Hisamine Kobayashi, Yusuke Shibui, Naohiko Inoue, Takahiro Narita, Masaki Hashimoto, Takuya Seki, Asuka Takumi |
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| Rok vydání: | 2020 |
| Předmět: |
NOAEL
No-observed-adverse-effect level Health Toxicology and Mutagenesis Wolffia globose 010501 environmental sciences Toxicology medicine.disease_cause 01 natural sciences Wolffia 03 medical and health sciences 0302 clinical medicine Animal science lcsh:RA1190-1270 Wolffia globosa Subchronic toxicity medicine Dry matter lcsh:Toxicology. Poisons 0105 earth and related environmental sciences biology Mankai biology.organism_classification Blood chemistry Toxicity Micronucleus test Rat Genotoxicity 030217 neurology & neurosurgery |
| Zdroj: | Toxicology Reports, Vol 7, Iss, Pp 1233-1241 (2020) |
| ISSN: | 2214-7500 |
| DOI: | 10.1016/j.toxrep.2020.09.006 |
| Popis: | Wolffia is a genus of protein-rich aquatic plants. Mankai, a cultivated strain of Wolffia globosa, contains more than 40 % protein based on dry matter evaluation. Furthermore, Mankai is nutritionally excellent as a food material, and is expected to be applicable to various products as a substitute for animal protein. A battery of toxicological studies was conducted on the dried product of Mankai (Dry Mankai), with the expectation to utilize it as a raw material for food applications. Dry Mankai was not genotoxic in a bacterial reverse mutation test and in vitro micronucleus assay. In the subchronic toxicity study, rats were provided Dry Mankai in the diet at levels of 0 %, 5 %, 10 %, or 20 % (w/w), equivalent to 0, 3.18, 6.49, and 13.16 g/kg/day for males and 0, 3.58, 7.42, and 15.03 g/kg/day for females, respectively. No adverse effects that could be attributable to treatment were observed in clinical observations, body weight, food consumption, ophthalmology, hematology and blood chemistry, urinalysis, and macroscopic and microscopic findings. According to the repeated-dose study in rats, the no observed adverse effect level of Dry Mankai was 20 % (w/w) for both sexes (13.16 and 15.03 g/kg/day for males and females, respectively). |
| Databáze: | OpenAIRE |
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