Bovine A20 gene overexpression during bovine viral diarrhea virus-1 infection blocks NF-κB pathway in MDBK cells
| Autor: | Nivia Canales, Nicolas Maldonado, Cristopher Stepke, Fernanda Fredericksen, Carola Otth, Melina Villalba, Víctor H. Olavarría, Valentina Arriagada, Pablo Garcés |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
viruses Immunology Biology Kidney Virus Cell Line Immunomodulation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immune system Animals Gene silencing Interleukin 8 RNA Small Interfering Gene Tumor Necrosis Factor alpha-Induced Protein 3 Diarrhea Virus 1 Bovine Viral Interleukin-8 Immunity NF-kappa B Epithelial Cells NF-κB Virology Blot 030104 developmental biology Gene Expression Regulation chemistry Bovine Virus Diarrhea-Mucosal Disease Cattle Signal transduction Signal Transduction 030215 immunology Developmental Biology |
| Zdroj: | Developmental & Comparative Immunology. 77:23-29 |
| ISSN: | 0145-305X |
| DOI: | 10.1016/j.dci.2017.07.019 |
| Popis: | Viruses have developed cellular strategies to ensure progeny survival. One of the most interesting is immune camouflage, where the virus triggers a controlled-intensity immune response that prevents total destruction of the infected cell, thus “winning time” for the virus. This study explored the regulatory contexts of the bovine A20 gene during bovine viral diarrhea virus (BVDV)-1 infection, using IL-8 as an immune-response sentinel molecule. Assessments were conducted through RT-qPCR, Western blotting, gene silencing/overexpression, luciferase assays, and the use of pharmacological inhibitors, among other approaches. The results demonstrated that a) BVDV-1 increased A20 levels in Madin-Darby bovine kidney cells, b) increased A20 led to decreased IL-8 expression, and c) the virus affected the NF-κB signaling pathway. Collectively, these data identify bovine A20 as a strong regulator of immune marker expression. In conclusion, this is the first report on BVDV-1 modulating bovine IL-8 activation through the NF-κB/A20 pathway. |
| Databáze: | OpenAIRE |
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