DMPC Phospholipid Bilayer as a Potential Interface for Human Cystatin C Oligomerization: Analysis of Protein-Liposome Interactions Using NMR Spectroscopy
| Autor: | Przemyslaw Jurczak, Stefan Jurga, Aneta Szymańska, Igor Zhukov, Slawomir Lach, Paulina Czaplewska, Kosma Szutkowski |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
protein–liposome interaction
Circular dichroism Amyloid Phospholipid Filtration and Separation Fibril lcsh:Chemical technology Article Cell membrane 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine NMR spectroscopy medicine Chemical Engineering (miscellaneous) lcsh:TP1-1185 lcsh:Chemical engineering Lipid bilayer human cystatin C phospholipid 030304 developmental biology 0303 health sciences Liposome Process Chemistry and Technology lcsh:TP155-156 15N relaxation Membrane medicine.anatomical_structure chemistry liposome Biophysics DMPC 030217 neurology & neurosurgery |
| Zdroj: | Membranes, Vol 11, Iss 13, p 13 (2021) Membranes Volume 11 Issue 1 |
| ISSN: | 2077-0375 |
| Popis: | Studies revolving around mechanisms responsible for the development of amyloid-based diseases lay the foundations for the recognition of molecular targets of future to-be-developed treatments. However, the vast number of peptides and proteins known to be responsible for fibril formation, combined with their complexity and complexity of their interactions with various cellular components, renders this task extremely difficult and time-consuming. One of these proteins, human cystatin C (hCC), is a well-known and studied cysteine-protease inhibitor. While being a monomer in physiological conditions, under the necessary stimulus&mdash usually a mutation, it tends to form fibrils, which later participate in the disease development. This process can potentially be regulated (in several ways) by many cellular components and it is being hypothesized that the cell membrane might play a key role in the oligomerization pathway. Studies involving cell membranes pose several difficulties therefore, an alternative in the form of membrane mimetics is a very attractive solution. Here, we would like to present the first study on hCC oligomerization under the influence of phospholipid liposomes, acting as a membrane mimetic. The protein&ndash mimetic interactions are studied utilizing circular dichroism, nuclear magnetic resonance, and size exclusion chromatography. |
| Databáze: | OpenAIRE |
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