Isolation and Structural Characterization of Antioxidant Peptides from Degreased Apricot Seed Kernels
| Autor: | Huan-Huan Xue, Hai-Sheng Zhang, Huanxia Zhao, Jing Xue, Wanrui Xue, Yu-Han Sun, Xin-Shuai Zhao |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Proteases
Antioxidant Prunus armeniaca medicine.medical_treatment Peptide Antioxidants Hydrolysate Analytical Chemistry Hydrolysis 0404 agricultural biotechnology medicine Environmental Chemistry Amino Acid Sequence Peptide sequence Chromatography High Pressure Liquid Pharmacology chemistry.chemical_classification Chromatography Reverse-Phase Protease Chromatography Chemistry 04 agricultural and veterinary sciences 040401 food science Amino acid Seeds Peptides Agronomy and Crop Science Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 101:1661-1663 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.5740/jaoacint.17-0465 |
| Popis: | Background: The composition and sequence of amino acids have a prominent influence on the antioxidant activities of peptides. Objective: A series of isolation and purification experiments was conducted to explore the amino acid sequence of antioxidant peptides, which led to its antioxidation causes. Methods: The degreased apricot seed kernels were hydrolyzed by compound proteases of alkaline protease and flavor protease (3:2, u/u) to prepare apricot seed kernel hydrolysates (ASKH). ASKH were separated into ASKH-A and ASKH-B by dialysis bag. ASKH-B (MW < 3.5 kDa) was further separated into fractions by Sephadex G-25 and G-15 gel-filtration chromatography. Reversed-phase HPLC (RP-HPLC) was performed to separate fraction B4b into two antioxidant peptides (peptide B4b-4 and B4b-6). Results: The amino acid sequences were Val-Leu-Tyr-Ile-Trp and Ser-Val-Pro-Tyr-Glu, respectively. Conclusions: The results suggested that ASKH antioxidant peptides may have potential utility as healthy ingredients and as food preservatives due to their antioxidant activity. Highlights: Materials with regional characteristics were selected to explore, and hydrolysates were identified by RP-HPLC and matrix-assisted laser desorption ionization-time-of-flight-MS to obtain amino acid sequences. |
| Databáze: | OpenAIRE |
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