Detection of Chlamydiaceae and Chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic region
| Autor: | Darja Keše, Hadeel Alchalabi, Gilbert Greub, Nicole Borel, Nadine Schuerer, Elisabeth Stein, Aleksandra Inic-Kanada, Astrid Collingro, Talin Barisani-Asenbauer, Florian Tagini, Balgesa Elkheir Babiker, Ehsan Ghasemian |
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| Přispěvatelé: | University of Zurich, Barisani-Asenbauer, Talin |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Endemic Diseases Physiology Chlamydia trachomatis Eye medicine.disease_cause Chlamydiaceae Young adult Child Phylogeny Aged 80 and over Multidisciplinary Chlamydia biology Middle Aged Adolescent Adult Aged Child Preschool Chlamydia trachomatis/isolation & purification Chlamydia trachomatis/physiology Chlamydiaceae/isolation & purification Chlamydiaceae/physiology DNA Bacterial/analysis Eye/microbiology Female Humans Infant Trachoma/epidemiology Trachoma/microbiology Young Adult Trachoma Medicine Ocular surface DNA Bacterial Science Chlamydiae 10184 Institute of Veterinary Pathology Article 03 medical and health sciences Conjunctival diseases medicine 1000 Multidisciplinary Bacteria business.industry medicine.disease biology.organism_classification Pathogenicity eye diseases 030104 developmental biology 570 Life sciences business |
| Zdroj: | Scientific reports, vol. 8, no. 1, pp. 7432 Scientific Reports, Vol 8, Iss 1, Pp 1-16 (2018) Scientific Reports |
| Popis: | Trachoma, the leading infectious cause of blindness, is caused by Chlamydia trachomatis (Ct), a bacterium of the phylum Chlamydiae. Recent investigations revealed the existence of additional families within the phylum Chlamydiae, also termed Chlamydia-like organisms (CLOs). In this study, the frequency of Ct and CLOs was examined in the eyes of healthy Sudanese (control) participants and those with trachoma (case). We tested 96 children (54 cases and 42 controls) and 93 adults (51 cases and 42 controls) using broad-range Chlamydiae and Ct-specific (omcB) real-time PCR. Samples positive by broad-range Chlamydiae testing were subjected to DNA sequencing. Overall Chlamydiae prevalence was 36%. Sequences corresponded to unclassified and classified Chlamydiae. Ct infection rate was significantly higher in children (31.5%) compared to adults (0%) with trachoma (p |
| Databáze: | OpenAIRE |
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