Functional diversity of isoprenoid lipids in Methylobacterium extorquens PA1
| Autor: | Maren Gnädig, Carlos Santana-Molina, Gesa Martens, Ngoc Anh Nguyen, Sandra Rizk, Meina Neumann-Schaal, Damien P. Devos, Petra Henke, James P. Sáenz |
|---|---|
| Přispěvatelé: | Federal Ministry of Education and Research (Germany), Volkswagen Foundation |
| Rok vydání: | 2021 |
| Předmět: |
Squalene
Hopanoids Membrane permeability C30 carotenoids Microbiology chemistry.chemical_compound Phytoene Methylobacterium extorquens Molecular Biology Carotenoid Phylogeny Sequence Deletion chemistry.chemical_classification biology Planctomycetes organic chemicals food and beverages biology.organism_classification Carotenoids Biosynthetic Pathways Isoprenoid lipids Oxidative Stress Bacterial Outer Membrane chemistry Biochemistry Gene Knockdown Techniques Geranylgeranyl-Diphosphate Geranylgeranyltransferase Bacterial membranes Bacterial outer membrane Oxidoreductases |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1365-2958 |
| Popis: | © 2021 The Authors Hopanoids and carotenoids are two of the major isoprenoid-derived lipid classes in prokaryotes that have been proposed to have similar membrane ordering properties as sterols. Methylobacterium extorquens contains hopanoids and carotenoids in their outer membrane, making them an ideal system to investigate the role of isoprenoid lipids in surface membrane function and cellular fitness. By genetically knocking out hpnE and crtB we disrupted the production of squalene and phytoene in M. extorquens PA1, which are the presumed precursors for hopanoids and carotenoids respectively. Deletion of hpnE revealed that carotenoid biosynthesis utilizes squalene as a precursor resulting in pigmentation with a C30 backbone, rather than the previously predicted canonical C40 phytoene-derived pathway. Phylogenetic analysis suggested that M. extorquens may have acquired the C30 pathway through lateral gene transfer from Planctomycetes. Surprisingly, disruption of carotenoid synthesis did not generate any major growth or membrane biophysical phenotypes, but slightly increased sensitivity to oxidative stress. We further demonstrated that hopanoids but not carotenoids are essential for growth at higher temperatures, membrane permeability and tolerance of low divalent cation concentrations. These observations show that hopanoids and carotenoids serve diverse roles in the outer membrane of M. extorquens PA1. This work was supported by the B CUBE, TU Dresden, a German Federal Ministry of Education and Research BMBF grant (to J.S., project 03Z22EN12), and a VW Foundation “Life” grant (to J.S., project 93090). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text