Egg yolk anti-BfpA antibodies as a tool for recognizing and identifying enteropathogenic Escherichia coli
| Autor: | Manoel Barral-Netto, Albert Schriefer, V. M. Quintana‐Flores, Enrique Medina-Acosta, C. M. C. De Almeida, W. Dias da Silva |
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| Rok vydání: | 2003 |
| Předmět: |
DNA
Bacterial Diarrhea Immunology Virulence Immunoglobulins In Vitro Techniques Pilus Bacterial Adhesion Microbiology law.invention law Escherichia coli Animals Humans Enteropathogenic Escherichia coli Immunoadsorption Escherichia coli Infections Ovum biology Base Sequence Escherichia coli Proteins General Medicine biochemical phenomena metabolism and nutrition Virology Antibodies Bacterial Recombinant Proteins Recombinant DNA biology.protein bacteria Immunoglobulin Y Female Fimbriae Proteins Antibody Protein A Chickens HeLa Cells |
| Zdroj: | Scandinavian journal of immunology. 57(6) |
| ISSN: | 0300-9475 |
| Popis: | Enteropathogenic Escherichia coli (EPEC) is a major aetiological agent of childhood diarrhoea in developing countries. The structural repeating protein A subunit, BfpA, found in the bundle-forming pilus, is one of the virulent factors for EPEC pathogenesis. Recombinant BfpA in laying hens elicited sustained and vigorous antibody production. Immunoglobulin Y (IgY) anti-BfpA antibodies were recovered from egg yolk, purified and characterized. Immunoadsorption with whole extracts of the isogenic E. coli EPEC adherence factor (EAF) strain that lacks BfpA rendered the resulting IgY preparations capable of: (a) recognizing purified or recombinant BfpA proteins in a dose-dependent fashion; (b) blocking the colonization of HeLa cells by EPEC EAFþ, in vitro; (c) specifically identifying E. coli bearing EAFþ; and (d) inhibiting the growth of E. coli EAFþ but not the EAF strain. IgY anti-BfpA is potentially useful as a specific, low-cost immunobiological reagent to screen human faecal specimens for the presence of EPEC. |
| Databáze: | OpenAIRE |
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