Genome-wide characterization and expression profiling of NAC transcription factor genes under abiotic stresses in radish (Raphanus sativus L.)
| Autor: | Yang Xie, Everlyne M’mbone Muleke, Bernard Kinuthia Karanja, Yan Wang, Liwang Liu, Bashir Mohammed Jabir, Xianwen Zhu, Liang Xu, Wanwan Cheng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Raphanus lcsh:Medicine General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound Genome-wide analysis Arabidopsis Abscisic acid Gene Transcription factor Expression profiling Genetics biology NAC Abiotic stress General Neuroscience lcsh:R food and beverages General Medicine biology.organism_classification Gene expression profiling 030104 developmental biology chemistry 13. Climate action Radish General Agricultural and Biological Sciences Transcription Factor Gene |
| Zdroj: | PeerJ, Vol 5, p e4172 (2017) |
| ISSN: | 2167-8359 |
| Popis: | NAC (NAM, no apical meristem; ATAF,Arabidopsistranscription activation factor and CUC, cup-shaped cotyledon) proteins are among the largest transcription factor (TF) families playing fundamental biological processes, including cell expansion and differentiation, and hormone signaling in response to biotic and abiotic stresses. In this study, 172 RsNACs comprising 17 membrane-bound members were identified from the whole radish genome. In total, 98RsNACgenes were non-uniformly distributed across the nine radish chromosomes.In silicoanalysis revealed that expression patterns of severalNACgenes were tissue-specific such as a preferential expression in roots and leaves. In addition, 21 representativeNACgenes were selected to investigate their responses to heavy metals (HMs), salt, heat, drought and abscisic acid (ABA) stresses using real-time polymerase chain reaction (RT-qPCR). As a result, differential expressions among these genes were identified whereRsNAC023andRsNAC080genes responded positively to all stresses except ABA, whileRsNAC145responded more actively to salt, heat and drought stresses compared with other genes. The results provides more valuable information and robust candidate genes for future functional analysis for improving abiotic stress tolerances in radish. |
| Databáze: | OpenAIRE |
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