Mycobacterium bovis AN5 antigens vary in their ability to induce nitric oxide production in blood monocytes of experimentally infected cattle
| Autor: | S.N. Joardar, Tapas Kumar Goswami, G. C. Ram |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Mycobacterium bovis Antigens Bacterial General Veterinary biology Monocyte Immunology biology.organism_classification Nitric Oxide Peripheral blood mononuclear cell In vitro Monocytes Nitric oxide Microbiology chemistry.chemical_compound medicine.anatomical_structure chemistry Antigen medicine Macrophage Animals Cattle Nitrite Tuberculosis Bovine |
| Zdroj: | Veterinary immunology and immunopathology. 93(1-2) |
| ISSN: | 0165-2427 |
| Popis: | Reactive nitrogen intermediates (RNI) are the principal effector molecules of activated monocyte/macrophage populations, responsible for killing and inhibiting the growth of virulent mycobacteria. In vitro nitrite production by blood monocytes of cattle inoculated with live Mycobacterium bovis AN5 was assessed from 0 day through 45 weeks post inoculation (PI). High in vitro nitrite production was observed at the 8th and 12th weeks PI in sensitized cattle but reactivity had fallen by the 20th week PI. To assess the in vitro nitrite producing ability of monocytes induced by individual polypeptides within culture filtrate antigens (CFA) of M. bovis AN5, cellular blotting was performed using peripheral blood mononuclear cells (PBMC) at the 12th week PI. It was observed that polypeptides of MW 70, 65, 60, 25, 24 and 22 kDa of CFA induced high nitrite production by blood monocytes while many polypeptides had little or no effect. |
| Databáze: | OpenAIRE |
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