Methodological considerations when conductingin vitro, air–liquid interface exposures to engineered nanoparticle aerosols
| Autor: | Nastassja Lewinski, Lynn E. Secondo, Nathan J. Liu |
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| Rok vydání: | 2016 |
| Předmět: |
Dose metrics
Nanotechnology 02 engineering and technology 010501 environmental sciences Toxicology Models Biological 01 natural sciences Toxicity Tests Humans Dosimetry 0105 earth and related environmental sciences Aerosols Titanium Air Pollutants Inhalation Exposure Air liquid interface Chemistry 021001 nanoscience & nanotechnology Engineered nanoparticles In vitro Nanotoxicology Nanoparticles Engineered Nanoparticle Zinc Oxide 0210 nano-technology Biomedical engineering |
| Zdroj: | Critical Reviews in Toxicology. 47:225-262 |
| ISSN: | 1547-6898 1040-8444 |
| Popis: | Little consistency exists in the methodology for toxicological testing of aerosolized nanoparticles used in in vitro, air-interfaced culture (AIC) exposure systems for engineered nanoparticles (ENPs) risk-assessment, preventing inter-laboratory comparisons to identify dose thresholds for adverse effects. These inconsistencies result from heterogeneity in particle types, exposure durations, exposure systems, and dose metrics reported. We screened 10,241 studies in the literature for toxicological assessment of ENPs, resulting in 110 publications included after meeting eligibility criteria. In this review, we critically analyzed methodology within these studies to answer whether: (1) the administered dose or the deposited dose correlated better with biological response, (2) a difference existed between various AIC exposure systems when depositing the same dose, (3) consistent results were generated for nanomaterials with similar physico-chemical properties, (4) the deposited dose in vitro correlated to the deposited dose in vivo, and (5) AIC studies reliably modeled acute toxicity in vivo. Methods used in delivering, measuring, and reporting ENP aerosol doses in vitro are summarized. Dosimetry and biological response comparisons of AIC, conventional suspensions, and in vivo exposures are discussed through case studies on silver, zinc oxide, titanium dioxide, and multi-walled carbon nanotube exposures. Finally, based on these findings, recommendations are offered for design of future AIC experiments to aid standardization and comparisons of results. |
| Databáze: | OpenAIRE |
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