Microtubule stabilization in vivo by nucleation-incompetent γ-tubulin complex
| Autor: | Kenneth E. Sawin, Andreas Anders |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Short Report
Biology Microtubules 03 medical and health sciences 0302 clinical medicine Microtubule Tubulin Schizosaccharomyces Cytoplasmic microtubule 030304 developmental biology Microtubule nucleation 0303 health sciences Tubulin complex Protein Stability Microtubule organizing center Cell Biology biology.organism_classification Fission yeast Gamma-tubulin Cell biology Treadmilling biology.protein Schizosaccharomyces pombe Proteins 030217 neurology & neurosurgery Microtubule-Organizing Center Protein Binding |
| Zdroj: | Journal of Cell Science Anders, A & Sawin, K E 2011, ' Microtubule stabilization in vivo by nucleation-incompetent gamma-tubulin complex ', Journal of Cell Science, vol. 124, no. 8, pp. 1207-1213 . https://doi.org/10.1242/jcs.083741 |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.083741 |
| Popis: | Although the fission yeast Schizosaccharomyces pombe contains many of the gamma-tubulin ring complex (gamma-TuRC)-specific proteins of the gamma-tubulin complex (gamma-TuC), several questions about the organizational state and function of the fission yeast gamma-TuC in vivo remain unresolved. Using 3 x GFP-tagged gamma-TuRC-specific proteins, we show here that gamma-TuRC-specific proteins are present at all microtubule organizing centers in fission yeast and that association of gamma-TuRC-specific proteins with the gamma-tubulin small complex (gamma-TuSC) does not depend on Mto1, which is a key regulator of the gamma-TuC. Through sensitive imaging in mto1 Delta mutants, in which cytoplasmic microtubule nucleation is abolished, we unexpectedly found that gamma-TuC incapable of nucleating microtubules can nevertheless associate with microtubule minus-ends in vivo. The presence of gamma-TuC at microtubule ends is independent of gamma-TuRC-specific proteins and strongly correlates with the stability of microtubule ends. Strikingly, microtubule bundles lacking gamma-TuC at microtubule ends undergo extensive treadmilling in vivo, apparently induced by geometrical constraints on plus-end growth. Our results indicate that microtubule stabilization by the gamma-TuC, independently of its nucleation function, is important for maintaining the organization and dynamic behavior of microtubule arrays in vivo. |
| Databáze: | OpenAIRE |
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