Genome-wide analysis of differentially expressed lncRNAs and mRNAs in primary gonadotrophin adenomas by RNA-seq
| Autor: | Wen Wang, Yazhuo Zhang, Hailong Li, Guidong Song, Zhenye Li, Chuzhong Li, Haoyuan Wang, Jianpeng Wang, Jiye Li, Zheng Zhao, Yazhou Miao, Guilin Li |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adenoma RNA-Seq Pituitary neoplasm Bioinformatics Transcriptome transcriptomics 03 medical and health sciences 0302 clinical medicine Beijing medicine Humans Gene Regulatory Networks Pituitary Neoplasms RNA Messenger Gene Oligonucleotide Array Sequence Analysis co-expression network business.industry Gene Expression Profiling medicine.disease Non-coding RNA ncRNA pathway analysis Gene Expression Regulation Neoplastic Gene expression profiling gonadotrophin adenoma Gene Ontology 030104 developmental biology Oncology 030220 oncology & carcinogenesis Female RNA Long Noncoding business Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | // Jiye Li 1 , Chuzhong Li 2 , Jianpeng Wang 3 , Guidong Song 1 , Zheng Zhao 1 , Haoyuan Wang 4 , Wen Wang 5 , Hailong Li 6 , Zhenye Li 5 , Yazhou Miao 1 , Guilin Li 1 , Yazhuo Zhang 2 1 Beijing Neurosurgical Institute, Capital Medical University, Tiantan Xili, Dongcheng District, Beijing, China 2 Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Beijing Institute for Brain Disorders Brain Tumor Center, China National Clinical Research Center for Neurological Diseases, Capital Medical University, Beijing, China 3 Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China 4 Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China 5 Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China 6 Department of Neurosurgery, Navy General Hospital, Beijing, China Correspondence to: Yazhuo Zhang, email: zyz2004520@yeah.net Keywords: gonadotrophin adenoma, transcriptomics, ncRNA, pathway analysis, co-expression network Received: November 09, 2016 Accepted: December 08, 2016 Published: December 15, 2016 ABSTRACT Recently, long non-coding RNAs (lncRNAs) have received increased research interest owing to their participation via distinct mechanisms in the biological processes of nonfunctional pituitary adenomas. However, changes in the expression of lncRNAs in gonadotrophin adenoma, which is the most common nonfunctional pituitary adenomas, have not yet been reported. In this study, we performed a genome-wide analysis of lncRNAs and mRNAs obtained from gonadotrophin adenoma patients’ samples and normal pituitary tissues using RNA-seq. The differentially expressed lncRNAs and mRNAs were identified using fold-change filtering. We identified 839 lncRNAs and 1015 mRNAs as differentially expressed. Gene Ontology analysis indicated that the biological functions of differentially expressed mRNAs were related to transcription regulator activity and basic metabolic processes. Ingenuity Pathway Analysis was performed to identify 64 canonical pathways that were significantly enriched in the tumor samples. Furthermore, to investigate the potential regulatory roles of the differentially expressed lncRNAs on the mRNAs, we constructed general co-expression networks for 100 coding and 577 non-coding genes that showed significantly correlated expression patterns in tumor cohort. In particular, we built a special sub-network of co-expression involving 186 lncRNAs interacting with 15 key coding genes of the mTOR pathway, which might promote the pathogenesis of gonadotrophin tumor. This is the first study to explore the patterns of genome-wide lncRNAs expression and co-expression with mRNAs, which might contribute to the molecular pathogenesis of gonadotrophin adenoma. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|