Differential distribution of IgA-protease genotypes in mucosal and invasive isolates of Haemophilus influenzae in Sweden
| Autor: | Victor Lindh, Guillaume Manat, Kristian Riesbeck, Timothy F. Murphy, Fredrik Resman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Haemophilus Infections Adolescent Genotype 030106 microbiology Respiratory System Biology medicine.disease_cause Polymerase Chain Reaction Microbiology Haemophilus influenzae lcsh:Infectious and parasitic diseases Pathogenesis Cohort Studies 03 medical and health sciences Young Adult Medical microbiology medicine Humans lcsh:RC109-216 Child Respiratory Tract Infections Aged Aged 80 and over Sweden Mucous Membrane IgA protease Serine Endopeptidases Middle Aged Exact test 030104 developmental biology Infectious Diseases medicine.anatomical_structure Parasitology Child Preschool Cohort Human infection Female Respiratory tract Research Article |
| Zdroj: | BMC Infectious Diseases, Vol 18, Iss 1, Pp 1-7 (2018) BMC Infectious Diseases |
| ISSN: | 1471-2334 |
| DOI: | 10.1186/s12879-018-3464-3 |
| Popis: | Background Several different IgA-proteases exist in Haemophilus influenzae. The variants have been suggested to play differential roles in pathogenesis, but there is limited information on their distribution in clinical isolates. The objective of this study was to investigate the distribution of IgA-protease genotypes in H. influenzae and assess the association between IgA-protease genotype and type of clinical infection. Methods We performed PCR-screening of the IgA-protease gene variants in two cohorts of clinical H. influenzae. The first cohort consisted of 177 isolates from individuals with respiratory tract infection in January 2010, 2011 and 2012. Information on age, gender and clinical infection was available in this cohort. The second cohort comprised 53 isolates, including NTHi from bloodstream, cerebrospinal fluid (CSF) and urogenital origin as well as encapsulated isolates respresenting all capsule types. We assessed associations between IgA protease genotype and clinical predictors using basic statistical tests of association as well as regression analysis. Results The igaB gene was found in 46% of isolates in the respiratory tract cohort, and no evident trend could be seen during the study years. However, the igaB gene was significantly less common among invasive isolates (19%), p = 0.003 (Fischer’s exact test), even when encapsulated isolates were excluded (21%), p = 0.012. A significantly negative association between bacteraemia and igaB genotype remained after adjusting for covariates. We did not identify a significant association between IgA-protease gene variants and type of respiratory tract infection, but isolates with an igaA2 genotype were overrepresented in pre-school children. Conclusions The distribution of IgA-protease gene variants in Swedish H. influenzae highlighted the widespread abundance of the igaB in isolates from cases of respiratory tract infection, but the igaB gene variant was significantly less common in invasive (bloodstream and CSF) isolates of H. influenzae compared with respiratory tract isolates. Electronic supplementary material The online version of this article (10.1186/s12879-018-3464-3) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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