Human chromosome 12 encodes a species-specific factor which increases human immunodeficiency virus type 1 tat-mediated trans activation in rodent cells
| Autor: | M Newstein, G Casey, P R Shank, E J Stanbridge |
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| Rok vydání: | 1990 |
| Předmět: |
Transcriptional Activation
Immunology Hybrid Cells Biology Microbiology Virus Cofactor Cell Line Mice Plasmid Species Specificity Transcription (biology) Virology Animals Humans Chromosome 12 Chromosomes Human Pair 12 Cell biology Cell culture Growth Hormone Insect Science Gene Products tat HIV-1 Trans-Activators biology.protein Chromosomes Human Pair 6 tat Gene Products Human Immunodeficiency Virus Trans-acting Research Article Plasmids |
| Zdroj: | Journal of Virology. 64:4565-4567 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | The human immunodeficiency virus type 1 (HIV-1) tat protein functions at a much lower level in rodent cells than in human cells. This species-specific difference in trans activation appears to be due to the lack of a functional homolog of a human cofactor for tat in rodent cells. Using HIV-1 long terminal repeat-driven human growth hormone as a reporter plasmid, we found that the tat-mediated trans activation functions at a level 5- to 20-fold lower in rodent cells than in human cells. Stable rodent-human hybrid cells containing only human chromosome 12 support a dramatically higher degree of trans activation. Thus, human chromosome 12 encodes a species-specific HIV-1 tat cofactor which, at least partially, restores high levels of tat-mediated trans activation. Chromosome 6 also appears to provide an additional factor which enhances HIV-1 tat-mediated trans activation in murine cells. |
| Databáze: | OpenAIRE |
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