Silica–gentamicin nanohybrids: combating antibiotic resistance, bacterial biofilms, and in vivo toxicity
| Autor: | Ulla Hynönen, Markus Linder, Wei He, Airi Palva, Katrina Nordström, Yaping Meng, Pezhman Mohammadi, Simo-Pekka Hannula, Qingling Feng, Dina A. Mosselhy |
|---|---|
| Přispěvatelé: | Veterinary Biosciences, Departments of Faculty of Veterinary Medicine |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Embryo Nonmammalian Antibiotics Pharmaceutical Science MRSA CONTROLLED-RELEASE 02 engineering and technology SILVER NANOPARTICLES SUSCEPTIBILITY medicine.disease_cause 0302 clinical medicine International Journal of Nanomedicine BONE RESEARCH Drug Discovery antibacterial and antibiofilm effects VITRO 1183 Plant biology microbiology virology Original Research 0303 health sciences Chemistry General Medicine Silicon Dioxide 021001 nanoscience & nanotechnology Anti-Bacterial Agents 3. Good health DIFFERENTIATION INFECTIONS Staphylococcus aureus Larva Toxicity Gentamicin Erratum SiO2 0210 nano-technology medicine.drug Methicillin-Resistant Staphylococcus aureus medicine.drug_class Biophysics Bioengineering Microbial Sensitivity Tests gentamicin Microbiology Biomaterials 03 medical and health sciences Antibiotic resistance In vivo Drug Resistance Bacterial Toxicity Tests Escherichia coli medicine Animals Humans ta216 ta215 030304 developmental biology ANTIMICROBIAL ACTIVITY SOL-GEL ZEBRAFISH Organic Chemistry Biofilm biochemical phenomena metabolism and nutrition 030104 developmental biology Biofilms nanotoxicity Nanoparticles Gentamicins 030217 neurology & neurosurgery |
| Zdroj: | Mosselhy, D A, He, W, Hynönen, U, Meng, Y, Mohammadi, P, Palva, A, Feng, Q, Hannula, S-P, Nordström, K & Linder, M B 2018, ' Silica–gentamicin nanohybrids : combating antibiotic resistance, bacterial biofilms, and in vivo toxicity ', International Journal of Nanomedicine, vol. 13, pp. 7939-7957 . https://doi.org/10.2147/IJN.S182611 International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| Popis: | Dina A Mosselhy,1–3 Wei He,4 Ulla Hynönen,5 Yaping Meng,6 Pezhman Mohammadi,1 Airi Palva,5 Qingling Feng,7 Simo-Pekka Hannula,2 Katrina Nordström,1 Markus B Linder11Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, Espoo, Finland; 2Department of Chemistry and Materials Science, School of Chemical Engineering, Aalto University, Espoo, Finland; 3Fish Diseases Department, Microbiological Unit, Animal Health Research Institute, Dokki, Giza 12618, Egypt; 4School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, People’s Republic of China; 5Department of Veterinary Biosciences, Division of Veterinary Microbiology and Epidemiology, University of Helsinki, Helsinki, Finland; 6State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, People’s Republic of China; 7State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, People’s Republic of China Introduction: Antibiotic resistance is a growing concern in health care. Methicillin-resistant Staphylococcus aureus (MRSA), forming biofilms, is a common cause of resistant orthopedic implant infections. Gentamicin is a crucial antibiotic preventing orthopedic infections. Silica–gentamicin (SiO2-G) delivery systems have attracted significant interest in preventing the formation of biofilms. However, compelling scientific evidence addressing their efficacy against planktonic MRSA and MRSA biofilms is still lacking, and their safety has not extensively been studied.Materials and methods: In this work, we have investigated the effects of SiO2-G nanohybrids against planktonic MRSA as well as MRSA and Escherichia coli biofilms and then evaluated their toxicity in zebrafish embryos, which are an excellent model for assessing the toxicity of nanotherapeutics.Results: SiO2-G nanohybrids inhibited the growth and killed planktonic MRSA at a minimum concentration of 500 µg/mL. SiO2-G nanohybrids entirely eradicated E. coli cells in biofilms at a minimum concentration of 250 µg/mL and utterly deformed their ultrastructure through the deterioration of bacterial shapes and wrinkling of their cell walls. Zebrafish embryos exposed to SiO2-G nanohybrids (500 and 1,000 µg/mL) showed a nonsignificant increase in mortality rates, 13.4±9.4 and 15%±7.1%, respectively, mainly detected 24 hours post fertilization (hpf). Frequencies of malformations were significantly different from the control group only 24 hpf at the higher exposure concentration.Conclusion: Collectively, this work provides the first comprehensive invivo assessment of SiO2-G nanohybrids as a biocompatible drug delivery system and describes the efficacy of SiO2-G nanohybrids in combating planktonic MRSA cells and eradicating E. coli biofilms.Keywords: SiO2, gentamicin, MRSA, antibacterial and antibiofilm effects, nanotoxicity, zebrafishErratumfor this paper has been published |
| Databáze: | OpenAIRE |
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