Comparison of non‐invasive tests with invasive tests in the diagnosis of celiac disease
| Autor: | Mihriban Yücel, Rukiye Berkem, Nilnur Eyerci, Ali Kudret Adiloğlu, Muzaffer Çaydere, Yasemin Derya Gülseren, Zuhal Dag, Levent Filik |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Adult Male medicine.medical_specialty Adolescent Tissue transglutaminase Biopsy Clinical Biochemistry Disease Human leukocyte antigen Gastroenterology Polymerase Chain Reaction Serology 03 medical and health sciences Young Adult 0302 clinical medicine Internal medicine medicine Immunology and Allergy Humans Serologic Tests Typing Pediatric gastroenterology Research Articles Aged Autoantibodies medicine.diagnostic_test biology business.industry Histocompatibility Testing Biochemistry (medical) Public Health Environmental and Occupational Health nutritional and metabolic diseases Hematology Hepatology Middle Aged Medical Laboratory Technology Celiac Disease 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Female business |
| Popis: | Background/aims Today, invasive diagnostic tests are necessary for definite diagnosis of adult celiac disease (CD). However, in selected children patients, the need for invasive tests is ceased. In this study, we evaluated adult patients according to the ESPGHAN (European Pediatric Gastroenterology Hepatology and Nutrition Society) criteria. Methods Thirty-nine patients (aged 17-66) with symptoms of CD were included. Serum samples were tested for total IgA, tTG-IgA (antitissue transglutaminase), tTG-IgG, DGP-IgA (antideamidated gliadin peptide), DGP-IgG, and EMA (endomysial antibodies). HLA-DQ typing was studied with PCR-SSP (sequence-specific primers) method. Biopsy samples were evaluated according to Marsh scoring. Results In CD patients, 71.4% (15/21) of the patients were diagnosed without biopsy according to the EPSGHAN criteria but when ESPGHAN's IgA tTG threshold value for children was taken into consideration (>200 IU/mL), the sensitivity decreased to 81%. Celiac disease diagnosed and control groups were compared in terms of HLA tissue types. DQ2.5 homozygous or DQ2.5/DQ2.2 was significantly higher in CD group, and DQ2- or DQ8-negative HLA tissue type was significantly higher in control group. Conclusion When serological tests, HLA typing, and clinical symptoms are all in favor of CD, biopsy may not be performed in selected adult CD patients. |
| Databáze: | OpenAIRE |
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