Repurposing Pilocarpine Hydrochloride for Treatment of Candida albicans Infections
| Autor: | Abeer M. Alghamdi, Monica Falleni, Christopher J. Nile, Oliver F. Anderson, Elisa Borghi, Emerenziana Ottaviano, Gordon Ramage, Christopher Delaney, Delfina Tosi, Gaetano Bulfamante, Daniela Cirasola, Giulia Morace |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cellular immunity Antifungal Agents muscarinic 030106 microbiology lcsh:QR1-502 repurposing Cholinergic Agonists Microbiology lcsh:Microbiology biofilm 03 medical and health sciences Immune system Candida albicans medicine Animals Immunologic Factors Receptors Cholinergic Molecular Biology Virulence biology fungi Candidiasis Drug Repositioning Pilocarpine Therapeutics and Prevention biochemical phenomena metabolism and nutrition biology.organism_classification QR1-502 Corpus albicans pilocarpine hydrochloride 3. Good health Lepidoptera Galleria mellonella Disease Models Animal 030104 developmental biology Cholinergic Acetylcholine Research Article medicine.drug |
| Zdroj: | mSphere, Vol 4, Iss 1 (2019) mSphere, Vol 4, Iss 1, p e00689-18 (2019) mSphere |
| ISSN: | 2379-5042 |
| DOI: | 10.1128/msphere.00689-18 |
| Popis: | Candida albicans is the most common human fungal pathogen with an estimated crude mortality rate of 40%. The ability of the organism to switch from the yeast to hyphal form and produce biofilms are important virulence factors. C. albicans infections are combatted by the host immune system. However, Candida triggers a strong inflammatory response that, if not appropriately regulated, can damage host tissues. Therefore, it is important that the host immune response eliminates the fungus but limits tissue damage. This study provides evidence that targeting cholinergic receptors cannot only curb the virulence of C. albicans by inhibiting filamentous growth and biofilm formation but can also appropriately regulate the host immune response to induce rapid clearance with limited damage to vital tissues. This article provides evidence that repurposing licensed drugs that target cholinergic receptors may offer novel therapeutic solutions for the prevention or treatment of fungal infections. Acetylcholine modulates the virulence of Candida albicans and regulates an appropriate immune response to infection in a Galleria mellonella infection model. Indeed, the evidence suggests that C. albicans possesses a functional cholinergic receptor that can regulate filamentous growth and biofilm formation. Furthermore, G. mellonella immune cell subsets possess repertories of cholinergic receptors which regulate an effective and appropriate cellular immune response to C. albicans infection. This study aimed to investigate the cholinergic receptor subtype involved in regulation of filamentous growth and biofilm formation by C. albicans and determine the roles of cholinergic receptors in modulation of G. mellonella immune cell subsets. The general muscarinic receptor agonist, pilocarpine hydrochloride, inhibited C. albicans biofilm formation and pathogenicity, a phenomenon that could be reversed using the general muscarinic receptor antagonist, scopolamine. Pilocarpine hydrochloride protected G. mellonella larvae from C. albicans infection via inhibition of C. albicans filamentation and appropriate regulation of cellular immunity. However, scopolamine abrogated the capacity of pilocarpine hydrochloride to protect G. mellonella larvae from C. albicans infection. Furthermore, acetylcholine and pilocarpine hydrochloride exhibited differential modulatory capabilities on Galleria mellonella hemocyte responses to C. albicans. The data in this article demonstrate that a muscarinic receptor modulates C. albicans filamentation and biofilm formation. Furthermore, the results suggest that G. mellonella hemocyte subsets possess unique repertoires of cholinergic receptors that regulate their differentiation, activation, and function in contrasting manners. Therefore, targeting cholinergic receptors by repurposing currently licensed cholinergic drugs may offer novel therapeutic solutions for the prevention or treatment of fungal infections. IMPORTANCE Candida albicans is the most common human fungal pathogen with an estimated crude mortality rate of 40%. The ability of the organism to switch from the yeast to hyphal form and produce biofilms are important virulence factors. C. albicans infections are combatted by the host immune system. However, Candida triggers a strong inflammatory response that, if not appropriately regulated, can damage host tissues. Therefore, it is important that the host immune response eliminates the fungus but limits tissue damage. This study provides evidence that targeting cholinergic receptors cannot only curb the virulence of C. albicans by inhibiting filamentous growth and biofilm formation but can also appropriately regulate the host immune response to induce rapid clearance with limited damage to vital tissues. This article provides evidence that repurposing licensed drugs that target cholinergic receptors may offer novel therapeutic solutions for the prevention or treatment of fungal infections. |
| Databáze: | OpenAIRE |
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