Encapsulation of porcine pancreatic islets within an immunoprotective capsule comprising methacrylated glycol chitosan and alginate

Autor: Matthew Oudshoorn, Kalyani Kathirgamanathan, Anna Louise Hillberg, Janice B. B. Lam
Rok vydání: 2014
Předmět:
Graft Rejection
Male
Materials science
Biocompatibility
Alginates
Swine
Ultraviolet Rays
Drug Compounding
Sus scrofa
Transplantation
Heterologous
Islets of Langerhans Transplantation
Biomedical Engineering
Biocompatible Materials
Capsules
Permeability
Polymerization
Biomaterials
Chitosan
Islets of Langerhans
Mice
chemistry.chemical_compound
Glucuronic Acid
In vivo
Materials Testing
Carbohydrate Conformation
medicine
Animals
Peritoneal Cavity
Cells
Cultured
Limulus Test
Molecular Structure
Foreign-Body Reaction
Hexuronic Acids
Pancreatic islets
technology
industry
and agriculture
Capsule
Hydrogels
Glucuronic acid
Microspheres
Transplantation
medicine.anatomical_structure
chemistry
Self-healing hydrogels
Methacrylates
Female
Peptides
Hydrophobic and Hydrophilic Interactions
Biomedical engineering
Zdroj: Journal of Biomedical Materials Research Part B: Applied Biomaterials. 103:503-518
ISSN: 1552-4981
1552-4973
DOI: 10.1002/jbm.b.33185
Popis: Encapsulation of cells in biocompatible polymer matrices represents a powerful tool for cell-based therapies and therapeutic delivery systems. This technology has successfully been used to deliver pancreatic islets to humans for the treatment of Type 1 diabetes. However, the clinical impact of this technology may be improved by reducing the inflammatory response brought on after implantation of capsules in vivo. Within this study a biocompatible polymeric delivery system combining alginate and photo-crosslinked methacrylated glycol chitosan (MGC) was developed. This approach involved encapsulating cells in calcium-alginate beads, coating with MGC and photo-polymerizing using UVA in the presence of photo-initiator (VA-086), resulting in the formation of capsules ∼600 µm in size. Crosslinking of the MGC outer wall allowed control over capsule swelling and improved the capsules overall properties. Capsule characterization demonstrated the stabilizing influence of polymerization and fluorescence imaging showed that the distribution of glycol chitosan is dependent on molecular weight. Good islet viability and insulin release was demonstrated in vitro over the course of a month, and in vivo transplantation of the capsules demonstrated good biocompatibility, particularly when compared with standard alginate/poly-l-ornithine/alginate capsules.
Databáze: OpenAIRE
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