High similarity of Trypanosoma cruzikDNA genetic profiles detected by LSSP-PCR within family groups in an endemic area of Chagas disease in Brazil
| Autor: | Luis Eduardo Ramirez, Sandra Maria Alkmim-Oliveira, Eliane Lages-Silva, Aluízio Prata, Cristiane Pontes Andrade, Henrique Borges Kappel, Dalmo Correia |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
Chagas disease Adult Male medicine.medical_specialty lcsh:Arctic medicine. Tropical medicine Adolescent Genotype lcsh:RC955-962 Trypanosoma cruzi Polymerase Chain Reaction Endemic area Young Adult LSSP-PCR Similarity (network science) Epidemiology parasitic diseases medicine Parasite hosting Humans Chagas Disease Child Genetics biology DNA Kinetoplast Genetic Variation DNA Protozoan Middle Aged biology.organism_classification medicine.disease Infectious Diseases Child Preschool Immunology Parasitology Female Indeterminate form Brazil |
| Zdroj: | Revista da Sociedade Brasileira de Medicina Tropical, Volume: 47, Issue: 5, Pages: 653-656, Published: OCT 2014 Revista da Sociedade Brasileira de Medicina Tropical, Vol 47, Iss 5, Pp 653-656 (2014) Revista da Sociedade Brasileira de Medicina Tropical v.47 n.5 2014 Revista da Sociedade Brasileira de Medicina Tropical Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT |
| Popis: | Introduction Determining the genetic similarities among Trypanosoma cruzi populations isolated from different hosts and vectors is very important to clarify the epidemiology of Chagas disease. Methods An epidemiological study was conducted in a Brazilian endemic area for Chagas disease, including 76 chronic chagasic individuals (96.1% with an indeterminate form; 46.1% with positive hemoculture). Results T. cruzi I (TcI) was isolated from one child and TcII was found in the remaining (97.1%) subjects. Low-stringency single-specific-primer-polymerase chain reaction (LSSP-PCR) showed high heterogeneity among TcII populations (46% of shared bands); however, high similarities (80-100%) among pairs of mothers/children, siblings, or cousins were detected. Conclusions LSSP-PCR showed potential for identifying similar parasite populations among individuals with close kinship in epidemiological studies of Chagas disease. |
| Databáze: | OpenAIRE |
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