Pervasive Protein Thermal Stability Variation during the Cell Cycle
| Autor: | Athanasios Typas, André Mateus, Maike Schramm, Frank Stein, Peer Bork, Christoph W. Müller, Mikhail M. Savitski, Florence Baudin, Nils Kurzawa, Isabelle Becher, Natalie Romanov, Dominic Helm, Marie-Therese Mackmull, Amparo Andres-Pons, Martin Beck |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Proteomics Hot Temperature Proteome Cell Mitosis RNA polymerase II Biology General Biochemistry Genetics and Molecular Biology Mass Spectrometry Article 03 medical and health sciences chemistry.chemical_compound medicine Cluster Analysis Humans Phosphorylation 030102 biochemistry & molecular biology Protein Stability Cell Cycle DNA Cell cycle Chromatin Assembly and Disassembly Chromatin 030104 developmental biology medicine.anatomical_structure chemistry Solubility Cardiovascular and Metabolic Diseases biology.protein Biophysics RNA Polymerase II thermal proteome profiling Protein Processing Post-Translational HeLa Cells |
| Zdroj: | Cell |
| ISSN: | 1097-4172 |
| Popis: | Summary Quantitative mass spectrometry has established proteome-wide regulation of protein abundance and post-translational modifications in various biological processes. Here, we used quantitative mass spectrometry to systematically analyze the thermal stability and solubility of proteins on a proteome-wide scale during the eukaryotic cell cycle. We demonstrate pervasive variation of these biophysical parameters with most changes occurring in mitosis and G1. Various cellular pathways and components vary in thermal stability, such as cell-cycle factors, polymerases, and chromatin remodelers. We demonstrate that protein thermal stability serves as a proxy for enzyme activity, DNA binding, and complex formation in situ. Strikingly, a large cohort of intrinsically disordered and mitotically phosphorylated proteins is stabilized and solubilized in mitosis, suggesting a fundamental remodeling of the biophysical environment of the mitotic cell. Our data represent a rich resource for cell, structural, and systems biologists interested in proteome regulation during biological transitions. Graphical Abstract Highlights • Proteome-wide variation of in situ protein thermal stability and solubility • Thermal stability of RNA Pol II varies across the cell cycle and is DNA dependent • Thermal profiling across the cell cycle delineates protein subcomplexes • Intrinsically disordered proteins are less prone to aggregation during mitosis A proteome-wide assessment of thermal stability and solubility during the eukaryotic cell cycle demonstrates pervasive variation in mitosis and G1. |
| Databáze: | OpenAIRE |
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