Muscovite Reverses Gastric Gland Atrophy and Intestinal Metaplasia by Promoting Cell Proliferation in Rats with Atrophic Gastritis
| Autor: | Miao Xu, Fang-shi Zhu, Qin Du, Yan Chen, Liangjing Wang, Shujie Chen, Qun-yan Zhou, Jian-min Si |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Gastritis
Atrophic Male Pathology medicine.medical_specialty Receptor ErbB-2 Atrophic gastritis Interleukin-1beta Biology Dinoprostone Rats Sprague-Dawley Atrophy Cell Line Tumor Proliferating Cell Nuclear Antigen Gastric glands Gastrins medicine Animals Humans Regeneration Cell Proliferation Metaplasia Tumor Necrosis Factor-alpha Cell growth Gastroenterology Intestinal metaplasia medicine.disease Rats ErbB Receptors medicine.anatomical_structure Gastric Mucosa Aluminum Silicates Adsorption |
| Zdroj: | Digestion. 79:79-91 |
| ISSN: | 1421-9867 0012-2823 |
| DOI: | 10.1159/000207489 |
| Popis: | Objective: To detect whether muscovite exerts its protective role in the progression of atrophic gastritis (AG) and evaluate the possible mechanism. Methods: AG rats were established and then randomly divided into groups administrated with different doses of muscovite for 8 weeks. Histological changes in gastric antrum and the number of parietal cells, chief cells, and G/D cells were observed. Serum gastrin and prostaglandin E2 (PGE2) were evaluated by enzyme-linked immunosorbent assay (ELISA). The expression of proliferating cell nuclear antigen (PCNA), epidermal growth factor receptor (EGFR), C-erbB-2, p21, p53, p16 and bcl-2 in gastric tissue were detected by immunohistochemistry. The concentrations of TNF-α and IL-1β secreted by THP-1 cells were detected when incubated with different doses of muscovite. Results: The grade of inflammation in muscovite groups was lower (p < 0.05), while the thickness and number of gastric glands were significantly elevated in muscovite groups (p < 0.01). The expression height of PCNA in the muscovite group was higher than in the AG group (p < 0.01). Immunohistochemistry results showed a positive expression rate of EGFR; p16 in muscovite-treated AG rats was increased (p < 0.05), while C-erbB-2 and p21 were decreased (p < 0.05). Serum gastrin and PGE2 were significantly elevated in the high-dose muscovite-treated rats (p < 0.05). In vitro studies showed that muscovite had a dose-dependent adsorption of TNF-α and IL-1β. Conclusion: Muscovite could reverse gastric gland atrophy and intestinal metaplasia by promoting cell proliferation and revitalization in gastric mucosa in AG rats. |
| Databáze: | OpenAIRE |
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