Capillary zone electrophoresis in polymer networks of polymerase chain reaction-amplified oligonucleotides: the case of congenital adrenal hyperplasia
| Autor: | Cecilia Gelfi, Monica Zanussi, Pier Giorgio Righetti, Antonia Orsi, Maurizio Ferrari, Paola Carrera |
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| Rok vydání: | 1994 |
| Předmět: |
Chromatography
Adrenal Hyperplasia Congenital Base Sequence Polymers Oligonucleotide Base pair Molecular Sequence Data Polyacrylamide Intercalation (chemistry) Oligonucleotides DNA General Chemistry Polymerase Chain Reaction Matrix (chemical analysis) chemistry.chemical_compound Electrophoresis Capillary electrophoresis chemistry Ethidium Humans Electrophoresis Polyacrylamide Gel Steroid 21-Hydroxylase Ethidium bromide Capillary Action Gene Deletion |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 657:201-205 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/0378-4347(94)80088-x |
| Popis: | The use of capillary zone electrophoresis (CZE) in polymer networks for the analysis of an 8 bp (base pair) deletion in congenital adrenal hyperplasia was investigated. Separations were performed in Tris-borate-EDTA buffer (pH 8.3) containing 6% liquid linear polyacrylamide as a sieving dynamic matrix and 10 microM ethidium bromide for improving DNA fragment separation. Easy analysis and detection of the 127 and 135 bp amplified fragments was accomplished. The capillary column can be used for50 analyses before degradation and loss of resolution. The results are comparable to those obtained by gel-slab zone electrophoresis in a 12%T, 4%C polyacrylamide matrix. The sensitivity, by simple UV absorption at 254 nm, is similar to that obtained in gel slabs by dye intercalation staining. |
| Databáze: | OpenAIRE |
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