Elevated neutrophil elastase and acrolein-protein adducts are associated with W256 regression
| Autor: | Morana Jaganjac, Suzana Borović, Kamelija Zarkovic, Marina Cindrić, Georg Waeg, Neven Zarkovic, Koji Uchida, Marija Poljak-Blazi, Ana Čipak, R. J. Schaur |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Immunology Biology Granulocyte Rats Sprague-Dawley Lipid peroxidation chemistry.chemical_compound granulocytes interleukin 17 lipid peroxidation neutrophil elastase tumor regression Malondialdehyde Tumor Microenvironment medicine Animals Immunology and Allergy Acrolein Rats Wistar Respiratory Burst Inflammation chemistry.chemical_classification Aldehydes Reactive oxygen species Cell Membrane Original Articles Rats Respiratory burst Transplantation medicine.anatomical_structure chemistry Neutrophil elastase Myeloperoxidase Disease Progression Fatty Acids Unsaturated biology.protein Lipid Peroxidation Leukocyte Elastase Reactive Oxygen Species Granulocytes |
| Zdroj: | Clinical and Experimental Immunology. 170:178-185 |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1111/j.1365-2249.2012.04639.x |
| Popis: | Summary The involvement of granulocytes in immune response against cancer is not well understood. Depending on the cytokine milieu in which they act and on their oxidative burst, granulocytes may play either an inhibitory or stimulatory role in tumour growth. Unsaturated fatty acids, essential components of cellular membranes and storage lipids, are susceptible to granulocyte-derived reactive oxygen species (ROS). ROS can induce lipid peroxidation (LPO) resulting in the destruction of biomembranes. Thus, murine W256 tumour progressing and tumour regressing animal models were used to study the involvement of plasma inflammatory mediators and oxidative burst of circulating granulocytes in malignant destruction and detrimental tumour growth. The involvement of LPO-derived aldehydes (i.e. acrolein, 4-hydroxy-2-nonenal and malondialdehyde) and myeloperoxidase (MPO) appearance in the granulocyte anti-cancer response were further evaluated. The results obtained revealed a significant increase in neutrophil elastase in animals with regressing tumour. Furthermore, the presence of MPO in tumour microenvironment was accompanied by the formation of acrolein only 5 h after tumour transplantation and its presence increased during tumour regression. Later, at an early stage of tumour regression, the presence of other LPO-derived aldehydes were also observed. The results obtained suggest that elevated neutrophil elastase and initiation of LPO may play an important role in the tumour development leading to tumour regression. |
| Databáze: | OpenAIRE |
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