Effect of 7-Methylsulfinylheptyl Isothiocyanate on the Inhibition of Melanogenesis in B16-F1 Cells

Autor:	Yeong Hee Cho, Do Sung Lim, A-Ju Kim, Jung Sup Lee, Jung Eun Park
Rok vydání:	2021
Předmět:	melanogenesis autophagy Tyrosinase Inflammation Article General Biochemistry Genetics and Molecular Biology ATG12 chemistry.chemical_compound whitening medicine B16-F1 lcsh:Science neoplasms skin aging Ecology Evolution Behavior and Systematics PI3K/AKT/mTOR pathway Chemistry Autophagy Paleontology Skin whitening Microphthalmia-associated transcription factor 7-MSI Cell biology Space and Planetary Science Isothiocyanate lcsh:Q medicine.symptom
Zdroj:	Life Volume 11 Issue 2 Life, Vol 11, Iss 162, p 162 (2021)
ISSN:	2075-1729
Popis:	Skin aging, characterized by hyperpigmentation, inflammation, wrinkles, and skin cancer, is influenced by intrinsic and extrinsic factors with synergistic effects. Autophagy maintains the homeostatic balance between the degradation, synthesis, and recycling of cellular proteins and organelles, and plays important roles in several cellular and biological processes, including aging. The compound 7-methylsulfinylheptyl isothiocyanate (7-MSI) is a sulfur-containing phytochemical produced by various plants, particularly cruciferous vegetables, with reported anti-inflammatory properties and a role in pathogen defense however, its effects on skin whitening have not been studied in detail. The purpose of this study was to observe the effects of 7-MSI on skin whitening and autophagy in cultured murine melanoma (B16-F1) cells. Western blotting was used to evaluate the impact of 7-MSI on melanogenesis-, tyrosinase-, and autophagy-associated proteins. The levels of the melanogenesis-associated protein’s microphthalmia-associated transcription factor (MITF) and tyrosinase and tyrosinase-related protein-1 were decreased by treatment with 7-MSI under melanogenesis induction. Melanin synthesis also decreased by approximately 63% after treatment with 7-MSI for 73 h, compared with that non-treated controls. In addition, autophagosome formation and the expression levels of the autophagy-related proteins mTOR, p-mTOR, Beclin-1, Atg12, and LC3 were higher in 7-MSI-treated B16-F1 cells than in non-treated cells. These results indicate that 7-MSI can inhibit melanin synthesis in B16-F1 cells by suppressing melanogenesis and autophagy activation and thus can potentially be used as a novel multifunctional cosmetic agent.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6dcef9d2fddcc0d8099700d97fcca885 https://pubmed.ncbi.nlm.nih.gov/33672463 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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