Thauera aminoaromatica MZ1T Identified as a Polyhydroxyalkanoate-Producing Bacterium within a Mixed Microbial Consortium

Autor: Jelmer Tamis, Janneke Krooneman, Dana I. Colpa, Marc C. A. Stuart, Wen Zhou, Gert-Jan Euverink, Jan Pier Wempe
Přispěvatelé: Bioconversion and fermentation technology, Products and Processes for Biotechnology, Groningen Biomolecular Sciences and Biotechnology, Stratingh Institute of Chemistry
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
Thauera
kunststoffen
Thauera aminoaromatica MZ1T
Population
Bioengineering
biopolymers
lcsh:Technology
01 natural sciences
Bioplastic
polyhydroxyalkanoate (PHA)
Polyhydroxyalkanoates
bio-renewable plastics
thauera aminoaromatica
Article
Polyhydroxybutyrate
03 medical and health sciences
chemistry.chemical_compound
010608 biotechnology
fossil fuel-based plastics
biodegradable plastics
Food science
microbiologie
education
fossiele brandstoffen
thauera aminoaromatica mz1t
lcsh:QH301-705.5
16S-rDNA analysis
030304 developmental biology
biodiversity
0303 health sciences
education.field_of_study
Growth medium
biology
lcsh:T
Chemistry
microbiology
transition
Biodegradable waste
Microbial consortium
biology.organism_classification
biologisch afbreekbare kunststoffen
polyhydroxybutyrate (PHB)
lcsh:Biology (General)
transitie
gemengd microbieel consortium
bio-hernieuwbare kunststoffen
mixed microbial consortium
Zdroj: Bioengineering
Bioengineering, 7(1). MDPI
AIMS Bioengineering, 7(1):19. AIMS Press
Bioengineering, Vol 7, Iss 1, p 19 (2020)
Volume 7
Issue 1
ISSN: 2306-5354
Popis: Polyhydroxyalkanoates (PHAs) form a highly promising class of bioplastics for the transition from fossil fuel-based plastics to bio-renewable and biodegradable plastics. Mixed microbial consortia (MMC) are known to be able to produce PHAs from organic waste streams. Knowledge of key-microbes and their characteristics in PHA-producing consortia is necessary for further process optimization and direction towards synthesis of specific types of PHAs. In this study, a PHA-producing mixed microbial consortium (MMC) from an industrial pilot plant was characterized and further enriched on acetate in a laboratory-scale selector with a working volume of 5 L. 16S-rDNA microbiological population analysis of both the industrial pilot plant and the 5 L selector revealed that the most dominant species within the population is Thauera aminoaromatica MZ1T, a Gram-negative beta-proteobacterium belonging to the order of the Rhodocyclales. The relative abundance of this Thauera species increased from 24 to 40% after two months of enrichment in the selector-system, indicating a competitive advantage, possibly due to the storage of a reserve material such as PHA. First experiments with T. aminoaromatica MZ1T showed multiple intracellular granules when grown in pure culture on a growth medium with a C:N ratio of 10:1 and acetate as a carbon source. Nuclear magnetic resonance (NMR) analyses upon extraction of PHA from the pure culture confirmed polyhydroxybutyrate production by T. aminoaromatica MZ1T.
Databáze: OpenAIRE
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