Intratumoral gene therapy versus intravenous gene therapy for distant metastasis control with 2-Diethylaminoethyl-Dextran Methyl Methacrylate Copolymer Non-Viral Vector-p53
| Autor: | George Koliakos, Antonios Sakkas, Haidong Huang, Kelly Domvri, Kokkona Kouzi-Koliakos, P. Zarogoulidis, P Le Pivert, Wolfgang Hohenforst-Schmidt, A Baliaka, A Chioti, E Koliakou, Leonidas Sakkas, Angeliki Cheva, K Tsakiridis, K. Zarogoulidis, E Siotou |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty Palliative care Genetic enhancement Medizin Antineoplastic Agents Methylmethacrylate Gene delivery Viral vector Carcinoma Lewis Lung Mice Pharmacokinetics Cell Line Tumor Genetics Carcinoma medicine Animals Neoplasm Metastasis Lung cancer Molecular Biology business.industry DEAE-Dextran Genetic Therapy Transfection medicine.disease Mice Inbred C57BL Cancer research Molecular Medicine Administration Intravenous Tumor Suppressor Protein p53 business Plasmids |
| Popis: | Lung cancer still remains to be challenged by novel treatment modalities. Novel locally targeted routes of administration are a methodology to enhance treatment and reduce side effects. Intratumoral gene therapy is a method for local treatment and could be used either in early-stage lung cancer before surgery or at advanced stages as palliative care. Novel non-viral vectors are also in demand for efficient gene transfection to target local cancer tissue and at the same time protect the normal tissue. In the current study, C57BL/6 mice were divided into three groups: (a) control, (b) intravenous and (c) intatumoral gene therapy. The novel 2-Diethylaminoethyl-Dextran Methyl Methacrylate Copolymer Non-Viral Vector (Ryujyu Science Corporation) was conjugated with plasmid pSicop53 from the company Addgene for the first time. The aim of the study was to evaluate the safety and efficacy of targeted gene therapy in a Lewis lung cancer model. Indeed, although the pharmacokinetics of the different administration modalities differs, the intratumoral administration presented increased survival and decreased distant metastasis. Intratumoral gene therapy could be considered as an efficient local therapy for lung cancer. |
| Databáze: | OpenAIRE |
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