Vascular smooth muscle cell apoptosis in aneurysmal, occlusive, and normal human aortas
| Autor: | Mitchell H. Goldman, Vincent L. Rowe, Robert L. Donnell, Tonya T. Reddick, Roger C. Carroll, Scott L. Stevens, Michael B. Freeman |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Aorta
Programmed cell death Pathology medicine.medical_specialty Vascular smooth muscle business.industry medicine.disease Aortic aneurysm chemistry.chemical_compound Terminal deoxynucleotidyl transferase chemistry Apoptosis medicine.artery medicine cardiovascular system Surgery Propidium iodide business Cardiology and Cardiovascular Medicine Immunostaining |
| Zdroj: | Journal of Vascular Surgery. 31(3):567-576 |
| ISSN: | 0741-5214 |
| DOI: | 10.1067/mva.2000.102847 |
| Popis: | Purpose: Apoptosis is a physiologic mechanism of cell death that regulates mass and architecture in many tissues. Apoptosis has been described as a feature in human vascular atherosclerosis and large vessel structural integrity. We examined the extent of vascular smooth muscle cell (VSMC) apoptosis in aneurysmal, occlusive, and normal human aortic tissue. Methods: Tissue samples of aneurysmal, occlusive, and normal human infrarenal aorta were evaluated. DNA fragmentation detection methods, immunohistochemistry, and DNA electrophoresis determined VSMC density, VSMC apoptosis, and apoptosis markers. Apoptotic cells and VSMC nuclei were counted with the use of computer-generated image analysis. Aortic subtypes were compared statistically by analysis of variance. Results: Seventeen aneurysmal, ten occlusive, and five normal human aortas were evaluated. By α1-actin immunostaining, VSMC density was least in aneurysmal aortas (271.8 ± 13.5 cells/high-power field [HPF]) compared with occlusive aorta (278.2 ± 39.4 cells/HPF) and normal aortas (291.0 ± 25.4 cells/HPF; P = not significant). Presence of apoptotic VSMCs was demonstrated by terminal deoxynucleotidyl transferase fragment end labeling and propidium iodide nuclear staining. VSMC apoptosis was greatest within aneurysmal aortas with 11.7 ± 1.5 cells/HPF compared with occlusive aortas with 3.3 ± 0.8 cells/HPF (P |
| Databáze: | OpenAIRE |
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