Involvement of Rho/ROCK signalling in small cell lung cancer migration through human brain microvascular endothelial cells
| Autor: | Zhi-Min Tan, Bo Li, Yu-Hua Chen, Wei-Dong Zhao, Wen-Gang Fang, Li Zhu |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Lung Neoplasms
Pyridines Biophysics Biology Protein Serine-Threonine Kinases Biochemistry chemistry.chemical_compound Structural Biology Cell Movement Cell Line Tumor Genetics Humans Phosphatidylinositol Carcinoma Small Cell Enzyme Inhibitors Neoplasm Metastasis Molecular Biology Rho-associated protein kinase Tight junction PI3K/AKT/mTOR pathway Protein kinase C Cytoskeleton Genes Dominant Regulation of gene expression rho-Associated Kinases Small cell lung cancer Rho/ROCK Brain Neoplasms Actin cytoskeleton reorganization Intracellular Signaling Peptides and Proteins Endothelial Cells Cell Biology Amides Cell biology Neoplasm Proteins Gene Expression Regulation Neoplastic chemistry Cell culture Brain endothelial cell Signal Transduction |
| Zdroj: | FEBS letters. 580(17) |
| ISSN: | 0014-5793 |
| Popis: | Small cell lung cancer (SCLC) cells migration across human brain microvascular endothelial cells (HBMECs) is an essential step of brain metastases. Here we investigated signalling pathways in HBMECs contributing to the process. Inhibition of endothelial Rho kinase (ROCK) with Y27632 and overexpression of ROCK dominant-negative mutant prevented SCLC cells, NCI-H209, transendothelial migration and the concomitant changes of tight junction. Conversely, inhibition of phosphatidylinositol 3-kinase (PI3K) and protein kinase C (PKC) had no effects. Furthermore, endothelial RhoA protein was activated during NCI-H209 cells transendothelial migration. Rho/ROCK participated in NCI-H209 cells transendothelial migration through regulating actin cytoskeleton reorganization. These results suggested that Rho/ROCK was required for SCLC cells transendothelial migration. |
| Databáze: | OpenAIRE |
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