Molecular heterogeneity of a Graves’ thyroid-infiltrating T cell population rich in CD8+ and γδ+ T cells
| Autor: | Pere Santamaria, J. J. Barbosa, C. Lewis |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Adolescent
CD8 Antigens T-Lymphocytes Endocrinology Diabetes and Metabolism Graves' disease T cell Molecular Sequence Data Population Peripheral blood mononuclear cell Endocrinology T-Lymphocyte Subsets medicine Humans Cytotoxic T cell Amino Acid Sequence education education.field_of_study Base Sequence business.industry T-cell receptor Receptors Antigen T-Cell gamma-delta T lymphocyte medicine.disease Graves Disease Phenotype medicine.anatomical_structure CD4 Antigens Immunology Interleukin-2 Female business CD8 |
| Zdroj: | Journal of Endocrinological Investigation. 16:913-920 |
| ISSN: | 1720-8386 0391-4097 |
| DOI: | 10.1007/bf03348958 |
| Popis: | We report here on the isolation and repertoire characterization of a Graves' thyroid T cell population which, unlike most Graves' thyroid T cell isolates reported before, was rich in CD4-CD8- T cells (16%, all TcR gamma delta+) and CD8+ T cells (75%) [TcR alpha beta+ (59%) and TcR gamma delta+ (16%)]. Only 7% of the isolated T cells were CD4+. By contrast,2% of peripheral blood mononuclear cells from the same patient were gamma delta+ and14% were CD8+. Sequence analysis of 18 TcR delta cDNAs prepared from these cells indicated the presence of at least 17 different gamma delta+ T cell clonotypes with molecularly heterogeneous antigen-binding site sequences. As opposed to most peripheral blood gamma delta+ T cells (express V delta 2 TcRs), 13 of the 17 different TcR delta clonotypes used a V delta 1 gene, although PCR amplification of TcR gamma- and TcR delta-specific cDNAs with V delta- and V gamma-gene family-specific oligonucleotides confirmed usage of all 4 V gamma and all 6 V delta families. Sequence analysis of 21 TcR alpha cDNAs from a TcR alpha-specific cDNA library indicated the presence of at least 12 different clonotypes, using 8 different V alpha gene families and heterogeneous antigen-binding site sequences. These results are in contrast with the selective V alpha gene usage reported for other intrathyroidal T cell subpopulations in autoimmune thyroid disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink