Inhibition of nuclease activity in Bacillus subtilis following infection with bacteriophage SP82G
Autor: | D. MacDonald Green, John J. King |
---|---|
Rok vydání: | 1977 |
Předmět: |
Nuclease
Deoxyribonucleases biology Chloramphenicol Cell Biophysics Cell Biology Bacillus subtilis biology.organism_classification Virus Replication Biochemistry Microbiology Bacteriophage Kinetics medicine.anatomical_structure Ribonucleases RNA Ribosomal DNA Viral biology.protein medicine Bacteriophages Molecular Biology medicine.drug |
Zdroj: | Biochemical and biophysical research communications. 74(2) |
ISSN: | 0006-291X |
Popis: | The total nuclease activity of cell extracts of B. subtilis was characterized and shown to decrease following infection with bacteriophage SP82G. This inhibition was maximal at 6 minutes after infection at 37° and 9 minutes after infection at 33°. The bulk (80%) of the inhibition was chloramphenicol sensitive but a small part (20%) was shown to be chloramphenicol insensitive. Extracts from infected cells were shown to have decreased nuclease activity on a variety of substrates. |
Databáze: | OpenAIRE |
Externí odkaz: |