Immune-Activated B Cells Are Dominant in Prostate Cancer

Autor: Aws Saudi, Viqar Banday, A. Ali Zirakzadeh, Martin Selinger, Jon Forsberg, Martin Holmbom, Johan Henriksson, Mauritz Waldén, Farhood Alamdari, Firas Aljabery, Ola Winqvist, Amir Sherif
Rok vydání: 2023
Předmět:
Zdroj: Cancers
Volume 15
Issue 3
Pages: 920
ISSN: 2072-6694
Popis: Simple Summary Contrary to the common belief that prostate cancer is an immune desert, our study shows tumor-associated B-cell responses in prostate cancer. We demonstrate mature and activated phenotypes of B cells with an increased frequency of effector plasmablasts in tumor-draining sentinel lymph nodes. These findings indicate a B-cell-specific antitumor immune response, emphasizing the importance of further trials targeting B cells in prostate cancer immunotherapy. B cells are multifaceted immune cells responding robustly during immune surveillance against tumor antigens by presentation to T cells and switched immunoglobulin production. However, B cells are unstudied in prostate cancer (PCa). We used flow cytometry to analyze B-cell subpopulations in peripheral blood and lymph nodes from intermediate-high risk PCa patients. B-cell subpopulations were related to clinicopathological factors. B-cell-receptor single-cell sequencing and VDJ analysis identified clonal B-cell expansion in blood and lymph nodes. Pathological staging was pT2 in 16%, pT3a in 48%, and pT3b in 36%. Lymph node metastases occurred in 5/25 patients (20%). Compared to healthy donors, the peripheral blood CD19(+) B-cell compartment was significantly decreased in PCa patients and dominated by naive B cells. The nodal B-cell compartment had significantly increased fractions of CD19(+) B cells and switched memory B cells. Plasmablasts were observed in tumor-draining sentinel lymph nodes (SNs). VDJ analysis revealed clonal expansion in lymph nodes. Thus, activated B cells are increased in SNs from PCa patients. The increased fraction of switched memory cells and plasmablasts together with the presence of clonally expanded B cells indicate tumor-specific T-cell-dependent responses from B cells, supporting an important role for B cells in the protection against tumors. Funding Agencies|SNIC through Uppsala Multidisciplinary Center for Advanced Computational Science (UPPMAX) [SNIC 2019/8-143]; Vetenskapsradet [2022-5-18]; Kempestiftelsen; Swedish government [RV-970150]; county councils [RV-970150]
Databáze: OpenAIRE
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