Protection Provided by a Gabexate Mesylate Thermo-Sensitive In Situ Gel for Rats with Grade III Pancreatic Trauma
| Autor: | Yukun Luo, Xiaoyan Li, Faqin Lv, Hanjing Gao, Yiru Wang, Xing-Guo Mei, Jie Tang, Qing Song, Shan Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
Pathology medicine.medical_specialty Gabexate H&E stain Thermo-sensitivity Grade III pancreatic trauma Apoptosis Therapeutics Abdominal Injuries Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Acinus In Situ Nick-End Labeling Medicine Animals Edema Pancreas Pancreatic duct TUNEL assay Hepatology business.industry Interleukin-6 Tumor Necrosis Factor-alpha Gastroenterology Ascites Lipase medicine.disease Rats medicine.anatomical_structure C-Reactive Protein Terminal deoxynucleotidyl transferase chemistry 030220 oncology & carcinogenesis Amylases Acute pancreatitis Main pancreatic duct 030211 gastroenterology & hepatology Original Article business Gels |
| Zdroj: | Gut and Liver |
| ISSN: | 2005-1212 1976-2283 |
| Popis: | Background/Aims This study investigated the protection provided by gabexate mesylate thermo-sensitive in-situ gel (GMTI) against grade III pancreatic trauma in rats. Methods A grade III pancreatic trauma model with main pancreatic duct dividing was established, and the pancreas anatomical diagram, ascites, and serum biochemical indices, including amylase, lipase, C-reactive protein (CRP), interleukin 6 (IL-6), and tumor necrosis factor-α (TNF-α), were examined. The pancreas was sliced and stained with hematoxylin eosin and subjected to terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. Results Ascites, serum amylase, lipase, CRP, IL-6, and TNF-α levels were significantly increased in the pancreas trauma (PT) groups with prolonged trauma time and were significantly decreased after GMTI treatment. The morphological structure of the pancreas was loose, the acinus was significantly damaged, the nuclei were irregular and hyperchromatic, and there was inflammatory cell invasion in the PT group compared to the control. After GMTI treatment, the morphological structure of the pancreas was restored, and the damaged acinus and inflammatory cell invasion were decreased compared to the PT group. Moreover, the cell apoptosis index was significantly increased in the PT group and restored to the same levels as the control group after GMTI treatment. Conclusions GMTI, a novel formulation and drug delivery method, exhibited specific effective protection against PT with acute pancreatitis therapy and has potential value as a minimally invasive adjuvant therapy for PT with acute pancreatitis. |
| Databáze: | OpenAIRE |
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