Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations
| Autor: | Jan Tavernier, Arne Soete, Matthieu Moisse, Radoje Drmanac, Irma Lemmens, Franki Speleman, Diether Lambrechts, Morgane Boone, Nico Callewaert, Nadine Van Roy, Yves Van de Peer, Leander Meuris, Joke Reumers, Stéphane Plaisance, Jason Chen, Yao-Cheng Lin |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
EXPRESSION
Genome instability Lineage (genetic) DNA Copy Number Variations Cell Survival Karyotype Molecular Sequence Data General Physics and Astronomy Biology Genome General Biochemistry Genetics and Molecular Biology Article Genomic Instability ADENOVIRUS LRP1B Transformation Genetic Humans TUMOR-SUPPRESSOR Gene Cell Proliferation Genetics Cryopreservation Multidisciplinary STABILITY Base Sequence MUTATIONS Cell growth Genome Human Gene Expression Profiling HEK 293 cells Biology and Life Sciences High-Throughput Nucleotide Sequencing DNA General Chemistry GENE RENAL-CANCER Adaptation Physiological Clone Cells Gene expression profiling HEK293 Cells FUMARATE HYDRATASE Human genome Transcriptome Plasmids |
| Zdroj: | Nature Communications NATURE COMMUNICATIONS |
| ISSN: | 2041-1723 |
| Popis: | The HEK293 human cell lineage is widely used in cell biology and biotechnology. Here we use whole-genome resequencing of six 293 cell lines to study the dynamics of this aneuploid genome in response to the manipulations used to generate common 293 cell derivatives, such as transformation and stable clone generation (293T); suspension growth adaptation (293S); and cytotoxic lectin selection (293SG). Remarkably, we observe that copy number alteration detection could identify the genomic region that enabled cell survival under selective conditions (i.c. ricin selection). Furthermore, we present methods to detect human/vector genome breakpoints and a user-friendly visualization tool for the 293 genome data. We also establish that the genome structure composition is in steady state for most of these cell lines when standard cell culturing conditions are used. This resource enables novel and more informed studies with 293 cells, and we will distribute the sequenced cell lines to this effect. The human embryonic kidney 293 (HEK293) cell lineage is widely used in cell biology and biotechnology. Here, the authors apply whole genome resequencing methods to characterise genomic variation in six HEK293 cell lines and suggest that this variation could affect experiments using these cell lines. |
| Databáze: | OpenAIRE |
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