Reversal of abnormal collagen production in Crohn's disease intestinal biopsies treated with regenerating agents
| Autor: | Jean-Pierre Caruelle, Jean-Pierre Gendre, A Sezeur, D Barritault, P. Kern, H. Mosnier, Jacques Cosnes, Laurent Beaugerie, M. Malafosse, Catherine Alexakis, D. Gallot |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Male medicine.medical_specialty Pathology Oligosaccharides Biology Collagen Type III Crohn Disease Pepsin Fibrosis In vivo Culture Techniques Internal medicine Biopsy medicine Humans Proline Intestinal Mucosa Glucocorticoids Aged medicine.diagnostic_test Inflammatory Bowel Disease Gastroenterology Dextrans Middle Aged medicine.disease In vitro Intestines Endocrinology biology.protein Female Collagen Ex vivo |
| Zdroj: | Gut. 53:85-90 |
| ISSN: | 0017-5749 |
| DOI: | 10.1136/gut.53.1.85 |
| Popis: | Background: Crohn’s disease (CD) is characterised by inflammation, muscle layer overgrowth, and collagenous fibrosis of the intestinal tract, with no effective therapy against collagen accumulation. Aims: We quantified production of collagen in resection specimens from normal and CD patients and investigated the effect of regenerating agents (RGTAs) on collagen production. RGTAs are chemically substituted dextrans engineered to mimic the growth factor protecting effects of heparan sulphates. RGTAs have been shown to enhance tissue repair in various in vivo models and to modulate in vitro collagen phenotype differentially according to their structure. Patients: We studied intestinal biopsies from two groups of CD patients: treated with glucocorticoids (CD-GC group: 10 patients) or not treated (CD group: seven patients), and from seven control patients. Methods: After 24 hours of ex vivo incubation with (3H) proline, collagen I, III, and V were extracted by pepsin and quantitatively separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Biosynthesis of each collagen type was quantified on radiolabelled isolated collagen. Results: Total intestinal collagen production in CD patients compared with controls was increased up to 3.5-fold overall (p |
| Databáze: | OpenAIRE |
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