Mycoviruses of Penicillium stoloniferum: influence of carbon–nitrogen nutrition upon replication
| Autor: | R. W. Detroy, P. E. Still, D. M. DeMarini |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Autolysis (biology)
Sucrose Lysis genetic structures Nitrogen Immunology Biology Virus Replication Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Genetics RNA Viruses Yeast extract Amino Acids Molecular Biology Penicillium General Medicine Carbon Virus Release Culture Media Oleic acid Glucose chemistry Viral replication Biochemistry Mycovirus circulatory and respiratory physiology |
| Zdroj: | Canadian Journal of Microbiology. 24:947-953 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/m78-157 |
| Popis: | Carbon–nitrogen ratio experiments indicate that limiting nutrition not only hinders Penicillium stoloniferum host proliferation but reduces total PsV-F and PsV-S virus replication. Results of C-N experiments show a pH-induced autolysis and virus release at minimal C levels. Maximal PsV-F levels and biomass were obtained with glucose and sucrose as C sources. Oleic acid also yielded high biomass and PsV-F yields. Yeast extract was an excellent N source; 2.83 g dry weight biomass and 87 A260 units PsV-F after 96 h of growth. Other nitrogen sources, including amino acids, supported only minimal growth and virus replication. The autolysis phenomenon is pH, not viral-induced. High C and N will support maximal growth and unrestricted virus replication with no cellular lysis. Under low C growth conditions, the replication of PsV-S is favored coupled with high pH and autolysis. |
| Databáze: | OpenAIRE |
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