Forensic genetic study of 29 Y-STRs in Korean population
| Autor: | Eunhye Kim, Han-Sol Kwon, Hyunchul Park, Si-Keun Lim, Ju Yeon Jung, Kyung-Hwa Park, Jihye Park, Yu-Li Oh, Dong-Sub Lee |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Forensic Genetics Population genetics Polymerase Chain Reaction Pathology and Forensic Medicine law.invention 03 medical and health sciences Forensic dna 0302 clinical medicine law Republic of Korea Medicine Humans 030216 legal & forensic medicine Allele Gene Polymerase chain reaction Genetics Chromosomes Human Y business.industry Korean population Genetic Variation Forensic science Issues ethics and legal aspects 030104 developmental biology Genetics Population Haplotypes Microsatellite business Microsatellite Repeats |
| Zdroj: | Legal medicine (Tokyo, Japan). 23 |
| ISSN: | 1873-4162 |
| Popis: | In this study, we compared two recently released commercial Y-chromosomal short tandem repeat (Y-STR) kits: the PowerPlex Y23 System (PPY23) and Yfiler® Plus PCR amplification kit (YPlus). We performed validation studies, including sensitivity, tolerance to PCR inhibitors, and mixture analysis, and a population genetics study using 306 unrelated South Korean males. PPY23 and YPlus showed similar sensitivity, but PPY23 showed higher tolerance to humic acid than YPlus. Furthermore, the detection rate of unique minor alleles called from male/male mixtures was higher for PPY23 than for YPlus. Comparing the newly added loci, the mean values of gene diversity for PPY23 and YPlus were 0.6715 and 0.8158, respectively. The discrimination capacity in the 306 unrelated South Korean males for PPY23 was 0.9837, and that for YPlus was 0.9935. These results will inform the selection of suitable Y-STR kits based on the purpose of forensic DNA analysis. |
| Databáze: | OpenAIRE |
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