Population Changes of Eubacteria, Sulfate-Reducing Bacteria and Methanogenic Archaea in an Anaerobic Reactor Processing Ethanol Distillery Vinasse
| Autor: | Márcia Helena Rissato Zamariolli Damianovic, Eloisa Pozzi, Adis Ivonne Terry Brown, Leslie Ester Abarzúa Ortiz, Homero Enrique Urrutia Briones, Eduardo Cleto Pires |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
education.field_of_study
Multidisciplinary biology Methanogenesis Chemistry lcsh:Biotechnology Population Vinasse Ribosomal RNA 16S ribosomal RNA biology.organism_classification lcsh:TP248.13-248.65 quantitative PCR ENGENHARIA HIDRÁULICA UASB reactor Food science Sulfidogenesis Sulfate-reducing bacteria DGGE education microbial characterization Bacteria Archaea |
| Zdroj: | Brazilian Archives of Biology and Technology v.62 2019 Brazilian Archives of Biology and Technology Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR Brazilian Archives of Biology and Technology, Vol 62 Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Brazilian Archives of Biology and Technology, Volume: 62, Article number: e19180268, Published: 13 JUN 2019 |
| Popis: | The microbiological characterization by molecular techniques (DGGE and quantitative PCR) of Archaea, Bacteria domain and sulfate-reducing bacteria (SRB) from a laboratory scale Up-flow Anaerobic Sludge Blanket (UASB) processing sugar cane vinasse was performed during the operational phase with increasing organic loads. The organic load removal efficiency was between 97% and 75% for volumetric organic loads (VOL) in the range of 0.6 to 15.4 kgCOD.m-3.d−1 and for higher VOL (until 27.0 kgCOD.m-3 .d−1) the removal efficiency decreased to 48%. Archaea represented the majority of the estimated population (107 copies of 16S RNA ribosomal gene. mL-1) followed by bacteria (106 copies of 16S RNA ribosomal gene. mL-1) and sulfate-reducing bacteria (SRB) (105 copies of dsrB gene. mL-1). There was a significant difference between the populations of Archaea and SRB with increasing VOL. DGGE profiles show differences among populations with increasing VOL, especially with respect to sulfate-reducing bacteria. The presence of SRB together with a high sulfate removal percentage (97%) of about 15 kgCOD.m-3d-1h, indicates the establishment of a sulfidogenic process. |
| Databáze: | OpenAIRE |
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