The needle in the haystack: Application of breast fine-needle aspirate samples to quantitative protein microarray technology
| Autor: | Armando C. Filie, Kevin Camphausen, Peter F. Lebowitz, Emanuel F. Petricoin, Lance A. Liotta, Andrea Abati, Jo Anne Zujewski, Virginia Espina, Amy Rapkiewicz, Julia Wulfkuhle |
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| Rok vydání: | 2007 |
| Předmět: |
Proteomics
Oncology Cancer Research Pathology medicine.medical_specialty Proteome Biopsy Fine-Needle Mammary gland Breast Neoplasms Breast cancer Prostate Cell Line Tumor Internal medicine Biopsy medicine Cluster Analysis Humans Breast medicine.diagnostic_test business.industry Reproducibility of Results Cancer Bayes Theorem Microarray Analysis Phosphoproteins medicine.disease medicine.anatomical_structure Fine-needle aspiration Docetaxel Protein microarray Female business Protein Kinases medicine.drug |
| Zdroj: | Cancer. 111:173-184 |
| ISSN: | 0008-543X |
| Popis: | BACKGROUND. There is an unmet clinical need for economic, minimally invasive procedures that use a limited number of cells for the molecular profiling of tumors in individual patients. Reverse-phase protein microarray (RPPM) technology has been applied successfully to the quantitative analysis of breast, ovarian, prostate, and colorectal cancers using frozen surgical specimens. METHODS. For this report, the authors investigated the novel use of RPPM technology for the analysis of both archival cytology aspirate smears and frozen fine-needle aspiration (FNA) samples. RPPMs were printed with 63 breast FNA samples that were obtained before, during, and after treatment from 21 patients who were enrolled in a Phase II trial of neoadjuvant capecitabine and docetaxel therapy for breast cancer. RESULTS. Based on an MCF7 cell line model of breast adenocarcinoma, the sensitivity of the RPPM detection method was in the femtomolar range with a coefficient of variance |
| Databáze: | OpenAIRE |
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