Analysis of the effects of Photodynamic therapy with Photodithazine on the treatment of 9l/lacZ cells, in vitro study
| Autor: | Juliana Ferreira-Strixino, Gabrielle dos Santos Vitorio, Rainara Moreno Sanches de Almeida, Letícia Corrêa Fontana, Juliana Guerra Pinto |
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| Rok vydání: | 2020 |
| Předmět: |
Gliosarcoma
medicine.medical_treatment PDZ domain Biophysics Brain tumor Photodynamic therapy Dermatology Mitochondrion law.invention chemistry.chemical_compound Confocal microscopy law Cell Line Tumor medicine Humans Pharmacology (medical) Glucosamine Photosensitizing Agents Chemistry medicine.disease Oncology Lac Operon Photochemotherapy Cytoplasm Chlorin Cancer research sense organs |
| Zdroj: | Photodiagnosis and photodynamic therapy. 34 |
| ISSN: | 1873-1597 |
| Popis: | Gliosarcoma is an aggressive brain tumor. Photodynamic Therapy (PDT) is a treatment that can be used for various cancers of the CNS. The aim of this study was to analyze the effects of PDT with Photodithazine (PDZ) in the treatment of gliosarcoma, using 9 L/lacZ cells and serial concentrations of 200 μg/mL to 3.1 μg/mL of PDZ. The samples were divided into two groups: dark and light (10 J/cm²). The PDZ was internalized along all the cytoplasmic extension. Viability tests demonstrated a reduction in viable cells after PDT. The production of ROS was concentration-dependent and PDZ was found in mitochondria and lysosomes, presenting a discrete connection with α-tubulin. However, this structure is likely damaged, evidenced by changes in the morphological analysis. Thus, according to the parameters of this study, PDZ proved to be an interesting PS in PDT for the treatment of gliosarcoma, with the inherent limitations of an in vitro study. |
| Databáze: | OpenAIRE |
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