Lack of induced mutagenesis in E. coli or human lymphoblast cell line upon long-term sub-culturing in medium from irradiated meat
| Autor: | Jyoti Tripathi, Sachin N. Hajare, Satyendra Gautam |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Meat
Radiological and Ultrasound Technology business.industry Cell Culture Techniques Dose-Response Relationship Radiation 04 agricultural and veterinary sciences Biology 040401 food science Lymphoblast cell line Cell biology Biotechnology Cell Line 0404 agricultural biotechnology Induced mutagenesis Mutagenesis Food Irradiation Escherichia coli Humans Radiology Nuclear Medicine and imaging Food irradiation Irradiation Lymphocytes Safety business Genome Bacterial |
| Zdroj: | International journal of radiation biology. 93(12) |
| ISSN: | 1362-3095 |
| Popis: | Current study was aimed to enhance the confidence of consumers as well as entrepreneurs towards food irradiation program.In this work, safety of high dose (25 kGy) irradiated meat samples (HDIMS) was ascertained by scoring mutation frequency through a long-term sub-culturing study in Escherichia coli MG1655 cells (ATCC 700926) up to 1500 generations (at 1%), 250 generations (at 5% and 10%) and human lymphoblast thymidine kinase heterozygote (TK6) cell line (ATCC CRL-8015) [at two gene loci, tkNone of the assays exhibited any induced mutation when analyzed at regular time intervals. RAPD analysis also did not indicate any change in its nucleotide sequence, ruling out the occurrence of any silent mutation. Thus, the present findings report absence of mutagenic effect of high dose irradiated meat samples. |
| Databáze: | OpenAIRE |
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